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However, no data about their morphology in immune\u2010associated disease conditions, and their relationship with lung infiltration, is available to date. In the present study, we compared the morphological features of <jats:styled-content style=\"fixed-case\">MFALC<\/jats:styled-content>s in 4\u2010month\u2010old male murine autoimmune disease models (<jats:styled-content style=\"fixed-case\">MRL<\/jats:styled-content>\/MpJ\u2010<jats:italic>lpr<\/jats:italic> mice and <jats:styled-content style=\"fixed-case\">BXSB<\/jats:styled-content>\/MpJ\u2010<jats:italic>Yaa<\/jats:italic> mice) with those of the corresponding control strains (<jats:styled-content style=\"fixed-case\">MRL<\/jats:styled-content>\/MpJ and <jats:styled-content style=\"fixed-case\">BXSB<\/jats:styled-content>\/MpJ, respectively). In addition, we analysed their correlation with lung infiltration. Furthermore, immunohistochemistry for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3, B220, Iba1, Gr1 and BrdU was performed to detect T cells and B cells, macrophages, granulocytes and proliferating cells, respectively. The spleen weight to body weight ratios and anti\u2010double\u2010stranded <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> autoantibody titres were found to be significantly higher in the autoimmune models than in the control strains. Furthermore, the autoimmune model presented prominent <jats:styled-content style=\"fixed-case\">MFALC<\/jats:styled-content>s, with a significantly greater ratio of lymphoid cluster area to total mediastinal fat tissue area, and more apparent diffused cellular infiltration into the lung lobes than the other studied strains. Higher numbers of T and B cells, macrophages and proliferating cells, but fewer granulocytes, were observed in the autoimmune models than in the control strains. Interestingly, a significant positive Pearson's correlation between the size of the <jats:styled-content style=\"fixed-case\">MFALC<\/jats:styled-content>s and the density of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3\u2010, B220\u2010 and Iba1\u2010positive cells in the lung was observed. Therefore, our data suggest a potentially important role for <jats:styled-content style=\"fixed-case\">MFALC<\/jats:styled-content>s in the progression of lung disease. However, further investigation is required to clarify the pathological role of <jats:styled-content style=\"fixed-case\">MFALC<\/jats:styled-content>s in lung disease, especially in inflammatory disorders.<\/jats:p>","DOI":"10.1111\/imm.12539","type":"journal-article","created":{"date-parts":[[2015,10,6]],"date-time":"2015-10-06T13:23:11Z","timestamp":1444137791000},"page":"30-40","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Comparative analysis of mediastinal fat\u2010associated lymphoid cluster development and lung cellular infiltration in murine autoimmune disease models and the corresponding normal control strains"],"prefix":"10.1111","volume":"147","author":[{"given":"Yaser Hosny Ali","family":"Elewa","sequence":"first","affiliation":[{"name":"Department of Histology and Cytology Faculty of Veterinary Medicine Zagazig University Zagazig Egypt"},{"name":"Laboratory of Anatomy Department of Biomedical 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