{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:26:58Z","timestamp":1791354418123,"version":"4.3.1"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,8,17]],"date-time":"2016-08-17T00:00:00Z","timestamp":1471392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["ANR\u201011\u2010LABX0024"],"award-info":[{"award-number":["ANR\u201011\u2010LABX0024"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We generated a <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8 T\u2010cell receptor (<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>) transnuclear (<jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content>) mouse specific to the L<jats:sup>d<\/jats:sup>\u2010restricted immunodominant epitope of <jats:styled-content style=\"fixed-case\">GRA<\/jats:styled-content>6 from <jats:italic>Toxoplasma gondii<\/jats:italic> as a source of cells to facilitate further investigation into the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8 T\u2010cell\u2010mediated response against this pathogen. The <jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content> T cells bound L<jats:sup>d<\/jats:sup>\u2010Gra6 tetramer and proliferated upon unspecific and peptide\u2010specific stimulation. The <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> beta sequence of the Gra6\u2010specific <jats:styled-content style=\"fixed-case\">TN CD<\/jats:styled-content>8 T cells is identical in its V\u2010 and J\u2010region to the <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>\u2010<jats:italic>\u03b2<\/jats:italic> harboured by a hybridoma line generated in response to Gra6 peptide. Adoptively transferred Gra6 <jats:styled-content style=\"fixed-case\">TN CD<\/jats:styled-content>8 T cells proliferated upon <jats:italic>Toxoplasma<\/jats:italic> infection <jats:italic>in vivo<\/jats:italic> and exhibited an activated phenotype similar to host <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8 T cells specific to Gra6. The brain of <jats:italic>Toxoplasma<\/jats:italic>\u2010infected mice carried Gra6 <jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content> cells already at day 8 post\u2010infection. Both Gra6 <jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content> mice as well as adoptively transferred Gra6 <jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content> cells were able to significantly reduce the parasite burden in the acute phase of <jats:italic>Toxoplasma<\/jats:italic> infection. Overall, the Gra6 <jats:styled-content style=\"fixed-case\">TN<\/jats:styled-content> mouse represents a functional tool to study the protective and immunodominant specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8 T\u2010cell response to <jats:italic>Toxoplasma<\/jats:italic> in both the acute and the chronic phases of infection.<\/jats:p>","DOI":"10.1111\/imm.12643","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T08:03:57Z","timestamp":1467705837000},"page":"270-279","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Transnuclear <scp>CD<\/scp>8 T cells specific for the immunodominant epitope Gra6 lower acute\u2010phase <i>Toxoplasma gondii<\/i> burden"],"prefix":"10.1111","volume":"149","author":[{"given":"Anna","family":"Sanecka","sequence":"first","affiliation":[{"name":"Host\u2013Toxoplasma Interaction Laboratory The Francis Crick Institute Mill Hill Laboratory London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagisa","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Host\u2013Toxoplasma Interaction Laboratory The Francis Crick Institute Mill Hill Laboratory London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie K.","family":"Dougan","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Jackson","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilabh","family":"Shastri","sequence":"additional","affiliation":[{"name":"Division of Immunology and Pathogenesis Department of Molecular and Cell Biology University of California Berkeley CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidde","family":"Ploegh","sequence":"additional","affiliation":[{"name":"Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology Cambridge MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Blanchard","sequence":"additional","affiliation":[{"name":"INSERM UMR1043 CNRS UMR5282 Universit\u00e9 de Toulouse\u2010UPS Centre de Physiopathologie de Toulouse Purpan (CPTP) Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva\u2010Maria","family":"Frickel","sequence":"additional","affiliation":[{"name":"Host\u2013Toxoplasma Interaction Laboratory The Francis Crick Institute Mill Hill Laboratory London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3128869"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.140.11.3943"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb08150.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1629"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00896.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000815"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178590"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1993.tb00616.x"},{"key":"e_1_2_7_11_1","first-page":"419","article-title":"Definitive identification of a gene that confers resistance against Toxoplasma cyst burden and encephalitis","volume":"85","author":"Brown CR","year":"1995","journal-title":"Immunology"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1128\/iai.62.1.221-228.1994","article-title":"Toxoplasma encephalitis in congenic B10 and BALB mice: impact of genetic factors on the immune response","volume":"62","author":"Deckert\u2010Schl\u00fcter M","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_7_13_1","first-page":"732","article-title":"A gene(s) within the H\u20102D region determines the development of toxoplasmic encephalitis in mice","volume":"74","author":"Suzuki Y","year":"1991","journal-title":"Immunology"},{"key":"e_1_2_7_14_1","doi-asserted-by":"crossref","first-page":"4649","DOI":"10.4049\/jimmunol.153.10.4649","article-title":"Gene determines development of toxoplasmic encephalitis in mice'","volume":"153","author":"Suzuki Y","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1128\/iai.19.2.416-420.1978","article-title":"Genetic control of murine resistance to Toxoplasma gondii","volume":"19","author":"Williams DM","year":"1978","journal-title":"Infect Immun"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.4049\/jimmunol.143.9.3031","article-title":"Genetic regulation of early survival and cyst number after peroral Toxoplasma gondii infection of A \u00d7 B\/B \u00d7 A recombinant inbred and B10 congenic mice","volume":"143","author":"McLeod R","year":"1989","journal-title":"J Immunol"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.2.966"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003449"},{"key":"e_1_2_7_19_1","article-title":"Transnuclear mice with pre\u2010defined T cell receptor specificities against Toxoplasma gondii obtained via SCNT","volume":"43","author":"Kirak O","year":"2010","journal-title":"J Vis Exp"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1086\/593019"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030590"},{"key":"e_1_2_7_22_1","first-page":"e2348","article-title":"Isolation of brain and spinal cord mononuclear cells using Percoll gradients","volume":"48","author":"Pino PA","year":"2011","journal-title":"J Vis Exp"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01862-06"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.695-702.2005"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2012.03.009"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12637"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1210273109"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-13-0047"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200137"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2014.48"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimm.12643","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.12643","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imm.12643","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.12643","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T19:29:09Z","timestamp":1696274949000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.12643"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,17]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1111\/imm.12643"],"URL":"https:\/\/doi.org\/10.1111\/imm.12643","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,17]]},"assertion":[{"value":"2016-05-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-06-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-08-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}