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A member of the Flaviviridae, ZikV is transmitted to humans by mosquitoes. It is related to other pathogenic vector\u2010borne flaviviruses including dengue, West Nile and Japanese encephalitis viruses, but produces a comparatively mild disease in humans. As a result of its epidemic outbreak and the lack of potential medication, there is a need for improved vaccine\/drugs. Computational techniques will provide further information about this virus. Comparative analysis of ZikV genomes should lead to the identification of the core characteristics that define a virus family, as well as its unique properties, while phylogenetic analysis will show the evolutionary relationships and provide clues about the protein's ancestry. Envelope glycoprotein of ZikV was obtained from a protein database and the most immunogenic epitope for T cells and B cells involved in cell\u2010mediated immunity, whereas B cells are primarily responsible for humoral immunity. We mainly focused on <jats:styled-content style=\"fixed-case\">MHC<\/jats:styled-content> class I potential peptides. <jats:italic><jats:styled-content style=\"fixed-case\">YRIMLSVHG<\/jats:styled-content><\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">VLIFLSTAV<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">MMLELDPPF<\/jats:styled-content><\/jats:italic>,<jats:italic> <jats:styled-content style=\"fixed-case\">GLDFSDLYY<\/jats:styled-content><\/jats:italic> are the most potent peptides predicted as epitopes for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells, respectively, whereas <jats:italic><jats:styled-content style=\"fixed-case\">MMLELDPPF<\/jats:styled-content><\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">GLDFSDLYY<\/jats:styled-content><\/jats:italic> had the highest <jats:styled-content style=\"fixed-case\">pMHC<\/jats:styled-content>\u2010I immunogenicity score and these are further tested for interaction against the <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content> molecules, using <jats:italic>in silico<\/jats:italic> docking techniques to verify the binding cleft epitope. However, this is an introductory approach to design an epitope\u2010based peptide vaccine against ZikV; we hope that this model will be helpful in designing and predicting novel vaccine candidates.<\/jats:p>","DOI":"10.1111\/imm.12656","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T08:42:53Z","timestamp":1470213773000},"page":"386-399","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["From ZikV genome to vaccine: <i>in silico<\/i> approach for the epitope\u2010based peptide vaccine against Zika virus envelope glycoprotein"],"prefix":"10.1111","volume":"149","author":[{"given":"Aftab","family":"Alam","sequence":"first","affiliation":[{"name":"Centre for Interdisciplinary Research in Basic Science Jamia Millia Islamia New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahnawaz","family":"Ali","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Basic Science Jamia Millia Islamia New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahzaib","family":"Ahamad","sequence":"additional","affiliation":[{"name":"Department of Biotechnology College of Engineering &amp; Technology IFTM Moradabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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