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Glycoprotein A repetitions predominant (GARP) expressed on activated CD4<jats:sup>+<\/jats:sup> T cells with suppressive activity has been established. This study aimed to investigate the frequency and function of circulating CD4<jats:sup>+<\/jats:sup><jats:bold>\u00a0<\/jats:bold>CD25<jats:sup>+<\/jats:sup>\u00a0GARP<jats:sup>+<\/jats:sup> regulatory T (Treg) cells in DCM. Forty\u2010five DCM patients and 46 controls were enrolled in this study. There was a significant increase in peripheral T helper type 1 (Th1) and Th17 number and their related cytokines [interferon\u2010<jats:italic>\u03b3<\/jats:italic> (IFN\u2010<jats:italic>\u03b3<\/jats:italic>), interleukin (IL\u201017)], and an obvious decrease in Treg number, transforming growth factor\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub> (TGF\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub>) levels and the expression of forkhead box P3 (FOXP3) and GARP in patients with DCM compared with controls. In addition, the suppressive function of CD4<jats:sup>+<\/jats:sup>\u00a0CD25<jats:sup>+<\/jats:sup>\u00a0GARP<jats:sup>+<\/jats:sup> Treg cells was impaired in DCM patients upon T\u2010cell receptor stimulation detected using CFSE dye. Lower level of TGF\u2010<jats:italic>\u03b2<\/jats:italic><jats:sub>1<\/jats:sub> and higher levels of IFN\u2010<jats:italic>\u03b3<\/jats:italic> and IL\u201017 detected using ELISA were found in supernatants of the cultured CD4<jats:sup>+<\/jats:sup>\u00a0CD25<jats:sup>+<\/jats:sup>\u00a0GARP<jats:sup>+<\/jats:sup> Treg cells in DCM patients compared with controls. Together, our results indicate that CD4<jats:sup>+<\/jats:sup>\u00a0CD25<jats:sup>+<\/jats:sup>\u00a0GARP<jats:sup>+<\/jats:sup> Treg cells are defective in DCM patients and GARP seems to be a better molecular definition of the regulatory phenotype. Therefore, it might be an attractive stategy to pay more attention to GARP in DCM patients.<\/jats:p>","DOI":"10.1111\/imm.12728","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T17:52:37Z","timestamp":1487267557000},"page":"291-303","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["CD4<sup>+<\/sup>\u00a0CD25<sup>+<\/sup>\u00a0GARP<sup>+<\/sup> regulatory T cells display a compromised suppressive function in patients with dilated cardiomyopathy"],"prefix":"10.1111","volume":"151","author":[{"given":"Yuzhen","family":"Wei","sequence":"first","affiliation":[{"name":"Laboratory of Cardiovascular Immunology Institute of Cardiology Union Hospital TongJi Medical College Huahzong University of Science and Technology Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunwu","family":"Yu","sequence":"additional","affiliation":[{"name":"Laboratory of 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