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However, little is known about the\n                    <jats:italic>B. tropicalis<\/jats:italic>\n                    epitopes recognized by T cells. Our aim was to identify the T\u2010cell epitopes in the major\n                    <jats:italic>B. tropicalis<\/jats:italic>\n                    allergen,\n                    <jats:italic>Blo t<\/jats:italic>\n                    5, and investigate the potential of the corresponding peptides to inhibit the allergic inflammatory lung response. C57\n                    <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\n                    \/6 mice were immunized with plasmid\n                    <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                    encoding\n                    <jats:italic>Blo t<\/jats:italic>\n                    5 and T\u2010cell epitopes identified using the interferon\u2010\n                    <jats:italic>\u03b3<\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">ELISPOT<\/jats:styled-content>\n                    assay with 15\u2010mer overlapping peptides. C57\n                    <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\n                    \/6 mice were sensitized with bone\u2010marrow\u2010derived dendritic cells (\n                    <jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content>\n                    ) pulsed with\n                    <jats:italic>Blo t<\/jats:italic>\n                    5 allergen followed by intranasal\n                    <jats:italic>Blo t<\/jats:italic>\n                    5 challenge. Two H\u20102\n                    <jats:sup>b<\/jats:sup>\n                    restricted epitopes (Bt5\n                    <jats:sub>76\u201390<\/jats:sub>\n                    and Bt5\n                    <jats:sub>106\u2013115<\/jats:sub>\n                    ) were recognized by\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    4 T cells specific for\n                    <jats:italic>Blo t<\/jats:italic>\n                    5, but no\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    8 epitopes were identified. In mice sensitized with\n                    <jats:italic>Blo t<\/jats:italic>\n                    5\u2010pulsed\n                    <jats:styled-content style=\"fixed-case\">BMDC<\/jats:styled-content>\n                    and challenged with intranasal\n                    <jats:italic>Blo t<\/jats:italic>\n                    5 Bt5\n                    <jats:sub>76\u201390<\/jats:sub>\n                    and Bt5\n                    <jats:sub>106\u2013115<\/jats:sub>\n                    , peptide\u2010specific\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    4 T cells were found to secrete the T helper type 2 cytokines interleukin\u20105 and interleukin\u201013. Intradermal administration of synthetic peptides encoding the identified T\u2010cell epitopes suppressed allergic airway inflammation to further allergen challenges. Hence, we have identified novel\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    4 T\u2010cell epitopes specific for\n                    <jats:italic>Blo t<\/jats:italic>\n                    5 and demonstrated that these peptides could be employed therapeutically to suppress the T\u2010cell response in a murine model of allergic airway inflammation.\n                  <\/jats:p>","DOI":"10.1111\/imm.12772","type":"journal-article","created":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T05:13:06Z","timestamp":1496639586000},"page":"344-355","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Blomia tropicalis<\/i>\n                    allergen 5 (\n                    <i>Blo t<\/i>\n                    5) T\u2010cell epitopes and their ability to suppress the allergic immune response"],"prefix":"10.1111","volume":"152","author":[{"given":"Kenneth H.","family":"Wong","sequence":"first","affiliation":[{"name":"Immunology Programme Life Sciences Institute Centre for Life Sciences National University of Singapore Singapore City Singapore"},{"name":"Department of Microbiology and Immunology Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qian","family":"Zhou","sequence":"additional","affiliation":[{"name":"Immunology Programme Life Sciences Institute Centre for Life Sciences National University of Singapore Singapore City Singapore"},{"name":"Department of Microbiology and Immunology Yong Loo Lin School of Medicine National University of Singapore Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nayana","family":"Prabhu","sequence":"additional","affiliation":[{"name":"Immunology Programme Life Sciences Institute Centre for Life Sciences National University of Singapore Singapore City 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