{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:05:56Z","timestamp":1790791556753,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2018,3]]},"abstract":"Summary<\/jats:title>Although one\u2010third of the world population is infected with Mycobacterium tuberculosis<\/jats:italic>, only 5\u201310% of the infected individuals will develop active tuberculosis (TB<\/jats:styled-content>) disease and the rest will remain infected with no symptoms, known as latent TB<\/jats:styled-content> infection (LTBI<\/jats:styled-content>). Identifying biomarkers that differentiate latent and active TB<\/jats:styled-content> disease enables effective TB<\/jats:styled-content> control, as early detection, treatment of active TB<\/jats:styled-content> and preventive treatment of individuals with LTBI<\/jats:styled-content> are crucial steps involved in TB<\/jats:styled-content> control. Here, we have evaluated the frequency of antigen\u2010specific memory and regulatory T (Treg) cells in 15 healthy household contacts (HHC<\/jats:styled-content>) and 15 pulmonary TB<\/jats:styled-content> patients (PTB<\/jats:styled-content>) to identify biomarkers for differential diagnosis of LTBI<\/jats:styled-content> and active TB<\/jats:styled-content>. Among all the antigens tested in the present study, early secretory antigenic target\u20106 (ESAT<\/jats:styled-content>\u20106) \u2010specific CD<\/jats:styled-content>4+<\/jats:sup> and CD<\/jats:styled-content>8+<\/jats:sup> central memory (Tcm) cells showed 93% positivity in HHC<\/jats:styled-content> and 20% positivity in PTB<\/jats:styled-content>. The novel test antigens Rv0753c and Rv0009 both displayed 80% and 20% positivity in HHC<\/jats:styled-content> and PTB<\/jats:styled-content>, respectively. In contrast to Tcm cells, effector memory T (Tem) cells showed a higher response in PTB<\/jats:styled-content> than HHC<\/jats:styled-content>; both ESAT<\/jats:styled-content>\u20106 and Rv0009 showed similar positivity of 80% in PTB<\/jats:styled-content> and 33% in HHC<\/jats:styled-content>. PTB<\/jats:styled-content> patients have a higher proportion of circulating antigen\u2010reactive Treg cells (CD<\/jats:styled-content>4+<\/jats:sup>\u00a0CD<\/jats:styled-content>25+<\/jats:sup>\u00a0FoxP3+<\/jats:sup>) than LTBI<\/jats:styled-content>. Rv2204c\u2010specific Treg cells showed maximum positivity of 73% in PTB<\/jats:styled-content> and 20% in HHC<\/jats:styled-content>. Collectively, our data conclude that ESAT<\/jats:styled-content>\u20106\u2010specific Tcm cells and Rv2204c\u2010specific Treg cells might be useful biomarkers to discriminate LTBI<\/jats:styled-content> from active TB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/imm.12833","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T18:57:43Z","timestamp":1504810663000},"page":"325-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Altered expression of antigen\u2010specific memory and regulatory T\u2010cell subsets differentiate latent and active tuberculosis"],"prefix":"10.1111","volume":"153","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2964-1632","authenticated-orcid":false,"given":"Balaji","family":"Pathakumari","sequence":"first","affiliation":[{"name":"Department of Immunology National Institute for Research in Tuberculosis (ICMR) Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santhi","family":"Devasundaram","sequence":"additional","affiliation":[{"name":"Department of Immunology National Institute for Research in Tuberculosis (ICMR) Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alamelu","family":"Raja","sequence":"additional","affiliation":[{"name":"Department of Immunology National Institute for Research in Tuberculosis (ICMR) Chennai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,17]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"World Health Organization(2015)Global tuberculosis report. 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