{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,7]],"date-time":"2025-06-07T16:27:51Z","timestamp":1749313671482,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T00:00:00Z","timestamp":1519084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004411","name":"Heart and Stroke Foundation of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>We previously showed that congenic bone marrow transplantation (<jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x) post myeloablation augmented tissue allograft survival in association with increased regulatory T (Treg) cells of both host and bone marrow donor origin. Regulatory B (Breg) cells can also modulate T\u2010cell immunity and B cells may be implicated in the development of Treg cells. Accordingly, we explored the effect of B\u2010cell depletion <jats:italic>in\u00a0vivo<\/jats:italic> on augmented graft survival post <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x. C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice received <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c skin allografts followed 7\u00a0days later by myeloablation using cyclophosphamide and busulphan. Mice then received T\u2010cell\u2010depleted bone marrow from <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45.1 congenic donors, and ongoing immunosuppression with rapamycin (to day 28 after BMTx). Control mice received cyclophosphamide and busulphan followed by rapamycin, but not congenic bone marrow. At different times post BMTx, mice received B\u2010cell\u2010depleting antibody treatment, and the effect on both skin graft survival, and induction of Treg cells was assessed. <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x resulted in significantly prolonged skin graft survival versus control mice, in association with attenuated donor\u2010specific alloreactivity relative to controls, increased splenic Treg cells and significantly diminished anti\u2010donor IgG. In mice receiving infusion of B\u2010depleting antibodies for 12\u00a0days from day 15 post <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x, both graft survival and Treg cell activity were diminished, particularly for functional Treg cells of donor origin. Adoptive transfer of Breg cells from mice harvested at 15\u00a0days post <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x prolonged survival in naive transplanted mice and increased Treg cell levels. Thus, autologous <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>x augmentation of graft survival is dependent in part upon a population of Breg cells that can modulate the function of donor\u2010derived Treg cells.<\/jats:p>","DOI":"10.1111\/imm.12895","type":"journal-article","created":{"date-parts":[[2018,1,16]],"date-time":"2018-01-16T18:53:55Z","timestamp":1516128835000},"page":"465-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Importance of B cells to development of regulatory T cells and prolongation of tissue allograft survival in recipients receiving autologous bone marrow transplantation"],"prefix":"10.1111","volume":"154","author":[{"given":"Reginald M.","family":"Gorczynski","sequence":"first","affiliation":[{"name":"Transplant Research Division University Health Network and Toronto General Hospital Toronto ON Canada"},{"name":"Department of Immunology Surgery University of 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