{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:54:54Z","timestamp":1790459694522,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572804"],"award-info":[{"award-number":["81572804"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The engagement of Epstein\u2013Barr virus (EBV)\u2010induced protein ligands in \u03b3\u03b4 T\u2010cell\u2010mediated anti\u2010EBV immunity, especially in EBV\u2010associated B\u2010cell malignancies, has not been fully elucidated. Previously we reported the overexpression of human MutS homologue 2 (hMSH2), a stress\u2010inducible protein ligand for human \u03b3\u03b4 T\u2010cells, on EBV\u2010transformed B lymphoblastic cell lines (B\u2010LCLs). In this study, we first generated EBV\u2010transformed B\u2010LCLs from peripheral blood mononuclear cells of healthy volunteers with B95\u20108 cellular supernatant and cyclosporine A. Secondly, we demonstrated the significantly elevated cell surface protein expression and mRNA transcription of <jats:italic>hMSH2<\/jats:italic> in EBV\u2010transformed B\u2010LCLs, 3D5 and EBV\u2010positive B lymphoma cell line Daudi and Raji. Thirdly, hMSH2\u2010mediated recognition of EBV\u2010transformed B malignant cells by human \u03b3\u03b4 T\u2010cells was confirmed by specific antibody blocking and siRNA interference. Both TCR\u03b3\u03b4 and NKG2D participated in hMSH2\u2010mediated recognition of EBV\u2010transformed B malignant cells. Furthermore, hMSH3 and hMSH6, the companion proteins of hMSH2, along with CD98, were found overexpressed on the surface of EBV\u2010transformed malignant B\u2010cells. We concluded that the induced overexpression of hMSH proteins might serve as early alerting biomarkers emerged in EBV\u2010related B\u2010cell malignances or as potential targets for establishing \u03b3\u03b4 T\u2010cell\u2010based therapeutic immunotherapies towards EBV infection.<\/jats:p>","DOI":"10.1111\/imm.12920","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T10:56:41Z","timestamp":1520420201000},"page":"673-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["EBV transformation induces overexpression of hMSH2\/3\/6 on B lymphocytes and enhances \u03b3\u03b4T\u2010cell\u2010mediated cytotoxicity via TCR and NKG2D"],"prefix":"10.1111","volume":"154","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4157-8653","authenticated-orcid":false,"given":"Yu\u2010mei","family":"Dai","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Medicine Guangzhou 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