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Currently, no clinically valid vaccine is available for this disease. In this study, we formulated <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and protein vaccines encoding <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A2, HLA\u2010A24 and HLA\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>1 restricted epitopes of Ca<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>2 against visceral leishmaniasis. We predicted the secondary and tertiary structures, surface properties, subcellular localization, potential binding sites and <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A2, HLA\u2010A24 and HLA\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>1 restricted epitopes of Ca<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>2. The best candidate CpG <jats:styled-content style=\"fixed-case\">ODN<\/jats:styled-content> (2395, M362, D\u2010<jats:styled-content style=\"fixed-case\">SL<\/jats:styled-content>03 or 685) was screened out as a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccine adjuvant. We also prepared Kmp\u201011 and Kmp\u201011\/Ca<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>2 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and protein vaccines, respectively, for comparison. <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice were immunized with a <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> prime\u2010protein boost immunization strategy and challenged with a newly isolated <jats:italic>Leishmania<\/jats:italic> strain from an individual with visceral leishmaniasis. The IgG antibody titers showed that our vaccine had strong immunogenicity with a long duration, especially cellular immunity. The spleen parasite burden of each group demonstrated that the Ca<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>2 vaccine had a certain immune protective effect on visceral leishmaniasis in <jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/c mice, and the amastigote reduction rate reached 76%. Preliminary safety tests confirmed the safety of the vaccine. Our work demonstrates that the <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010A2, HLA\u2010A24 and HLA\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>1 restricted epitope Ca<jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content>2 <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> prime\u2010protein boost vaccine may be a safe and effective epitope vaccine candidate against visceral leishmaniasis.<\/jats:p>","DOI":"10.1111\/imm.13007","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T23:26:26Z","timestamp":1538781986000},"page":"94-108","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["<scp>DNA<\/scp> prime\u2010protein boost vaccine encoding <scp>HLA<\/scp>\u2010A2, HLA\u2010A24 and HLA\u2010<scp>DR<\/scp>1 restricted epitopes of Ca<scp>NA<\/scp>2 against visceral 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