{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:08:43Z","timestamp":1791425323272,"version":"4.3.3"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,11,8]],"date-time":"2018-11-08T00:00:00Z","timestamp":1541635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007637","name":"Departamento Administrativo de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["111556933247"],"award-info":[{"award-number":["111556933247"]}],"id":[{"id":"10.13039\/100007637","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005278","name":"Universidad de Antioquia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005278","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Although dengue can progress to severe stages, the exact causes of this phenomenon are unknown; however, the possibility of monocyte participation is acknowledged. It has been suggested that monocyte subsets (classical, intermediate and non\u2010classical) play differential roles in dengue immunopathology. Therefore, we determined the count of monocyte subsets and obtained the clinical information of patients with dengue. We noted a significant decrease in the count of non\u2010classical monocytes in patients compared with controls. With this finding, we focused on studying the phenotype of non\u2010classical monocytes in the present study. An increase in activation and differentiation markers, such as CD64, CD86, the percentage of tumor necrosis factor\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    <jats:sup>+<\/jats:sup>\n                    cells and exposure of phosphatidylserine, were recorded in the non\u2010classical monocytes of patients compared with controls. Moreover, a significant decrease in the expression of CX3CR1 with a corresponding increase in the expressions of CCR2, CCR5, CD11b and CD54 was detected in the non\u2010classical monocytes of patients in comparison with that of the controls. Significant increases in the frequency of microparticles from endothelium and in the concentrations of interleukin\u20106 (IL\u20106), IL\u20108 and IL\u201010 were noted in the plasma of patients. These findings demonstrate that in patients with dengue, non\u2010classical monocytes are activated, exhibiting a phenotype associated with more differentiation, produces tumor necrosis factor\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    and has a profile of less endothelial surveillance closer to the cellular migration. These changes were associated with hepatic compromise, endothelial alteration and high concentration of circulating cytokines. Hence, alterations of non\u2010classical monocytes seem to be associated with the immunopathology of dengue infection.\n                  <\/jats:p>","DOI":"10.1111\/imm.13011","type":"journal-article","created":{"date-parts":[[2018,10,13]],"date-time":"2018-10-13T06:58:26Z","timestamp":1539413906000},"page":"147-163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Different phenotypes of non\u2010classical monocytes associated with systemic inflammation, endothelial alteration and hepatic compromise in patients with dengue"],"prefix":"10.1111","volume":"156","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2971-2506","authenticated-orcid":false,"given":"Juan S.","family":"Naranjo\u2010G\u00f3mez","sequence":"first","affiliation":[{"name":"Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica Facultad de Medicina Instituto de Investigaciones M\u00e9dicas Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Andr\u00e9s","family":"Castillo","sequence":"additional","affiliation":[{"name":"Grupo de Inmunovirolog\u00eda Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Medicina Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7791-9328","authenticated-orcid":false,"given":"Mauricio","family":"Rojas","sequence":"additional","affiliation":[{"name":"Unidad de Citometr\u00eda Facultad de Medicina Sede de Investigaci\u00f3n Universitaria Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7960-4162","authenticated-orcid":false,"given":"Berta N.","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Instituto Colombiano de Medicina Tropical Universidad CES Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3352-3652","authenticated-orcid":false,"given":"Francisco J.","family":"Diaz","sequence":"additional","affiliation":[{"name":"Grupo de Inmunovirolog\u00eda Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Medicina Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0885-1320","authenticated-orcid":false,"given":"Paula A.","family":"Velilla","sequence":"additional","affiliation":[{"name":"Grupo de Inmunovirolog\u00eda Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Medicina Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5697-5603","authenticated-orcid":false,"given":"Diana","family":"Casta\u00f1o","sequence":"additional","affiliation":[{"name":"Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica Facultad de Medicina Instituto de Investigaciones M\u00e9dicas Universidad de Antioquia UdeA Medell\u00edn Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61687-0"},{"key":"e_1_2_9_3_1","volume-title":"Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control","author":"WHO","year":"2009"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60572-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.06.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0036435"},{"key":"e_1_2_9_7_1","first-page":"658","article-title":"Human immune responses to dengue viruses","volume":"21","author":"Kurane I","year":"1990","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21051"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.03.028"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10340"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e3283324f80"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.03.010"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.08.012"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00105-06"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00322"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.38860"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005904"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.23307"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000790"},{"key":"e_1_2_9_20_1","first-page":"1445","article-title":"Secondary infection as a risk factor for dengue hemorrhagic fever\/dengue shock syndrome: an historical perspective and role of antibody\u2010dependent enhancement of infection","volume":"158","author":"Guzman MG","year":"2013","journal-title":"Adv Virol"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007475"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3017"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-403"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2011.04.002"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03224.x"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virep.2017.02.001"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03027.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2012.02.013"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00423"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-6-10"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038527"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20798"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015680"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12476"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28549"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2007.04.012"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep13886"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3014"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100008"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.68.48"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.055277-0"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762009000800005"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1142883"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02207-14"},{"key":"e_1_2_9_45_1","doi-asserted-by":"crossref","first-page":"R6","DOI":"10.1186\/ar2590","article-title":"Increased expression of Fc\u03b3RI\/CD64 on circulating monocytes parallels ongoing inflammation and nephritis in lupus","volume":"11","author":"Li Y","year":"2009","journal-title":"Arthritis Res Ther"},{"key":"e_1_2_9_46_1","first-page":"917143","article-title":"Vascular leakage in dengue hemorrhagic Fever is associated with dengue infected monocytes, monocyte activation\/exhaustion, and cytokines production","volume":"2015","author":"Chunhakan S","year":"2015","journal-title":"Int J Vasc Med"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-5-64"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.11.5588-5597.2002"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017484"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v11.i18.2754"},{"key":"e_1_2_9_51_1","doi-asserted-by":"crossref","first-page":"485","DOI":"10.4049\/jimmunol.148.2.485","article-title":"Transforming growth factor\u2010\u03b2 in synovial fluids modulates Fc gamma RII (CD16) expression on mononuclear phagocytes","volume":"148","author":"Wahl SM","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2015.1112687"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001130050197"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1997.tb05048.x"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-017-1226-4"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2264-2271.1998"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X13499773"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-326355"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimm.13011","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.13011","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T19:50:39Z","timestamp":1775245839000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.13011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,8]]},"references-count":57,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["10.1111\/imm.13011"],"URL":"https:\/\/doi.org\/10.1111\/imm.13011","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,8]]},"assertion":[{"value":"2018-03-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-10-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}