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Regulatory T (Treg) cells possess an immunosuppressive ability to inhibit effector T\u2010cell responses, and Notch ligand Jagged1 (Jag1) is implicated in Treg cell differentiation. In this study, we evaluated whether bone marrow\u2010derived <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s genetically engineered to express Jag1 (Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s) would affect the maturation and function of <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s <jats:italic>in vitro<\/jats:italic> and further investigated the immunoregulatory ability of Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s to manipulate T helper type 2 (Th2) \u2010mediated allergic asthma in mice. We produced Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s by adenoviral transduction. Overexpression of Jag1 by ovalbumin (<jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>) \u2010stimulated Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s exhibited increased expression of programmed cell death ligand 1 (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1) and <jats:styled-content style=\"fixed-case\">OX<\/jats:styled-content>40L molecules. Subsequently, co\u2010culture of these <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010pulsed Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s with allogeneic or syngeneic <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T cells promoted the generation of Foxp3<jats:sup>+<\/jats:sup> Treg cells, and blocking <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 using specific antibodies partially reduced Treg cell expansion. Furthermore, adoptive transfer of <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010pulsed Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s to mice with <jats:styled-content style=\"fixed-case\">OVA<\/jats:styled-content>\u2010induced asthma reduced allergen\u2010specific immunoglobulin E production, airway hyperresponsiveness, airway inflammation, and secretion of Th2\u2010type cytokines (interleukin\u20104, interleukin\u20105, and interleukin\u201013). Notably, an increased number of Foxp3<jats:sup>+<\/jats:sup> Treg cells associated with enhanced levels of transforming growth factor\u2010<jats:italic>\u03b2<\/jats:italic> production was observed in Jag1\u2010<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>\u2010treated mice. These data indicate that transgenic expression of Jag1 by <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s promotes induction of Foxp3<jats:sup>+<\/jats:sup> Treg cells, which ameliorated Th2\u2010mediated allergic asthma in mice. Our study supports an attractive strategy to artificially generate immunoregulatory <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s and provides a novel approach for manipulating Th2 cell\u2010driven deleterious immune diseases.<\/jats:p>","DOI":"10.1111\/imm.13021","type":"journal-article","created":{"date-parts":[[2018,11,12]],"date-time":"2018-11-12T14:13:58Z","timestamp":1542032038000},"page":"199-212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Jagged1\u2010expressing adenovirus\u2010infected dendritic cells induce expansion of Foxp3<sup>+<\/sup> regulatory T cells and alleviate T helper type 2\u2010mediated allergic asthma in mice"],"prefix":"10.1111","volume":"156","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3739-0631","authenticated-orcid":false,"given":"Chu\u2010Lun","family":"Lin","sequence":"first","affiliation":[{"name":"Graduate Institute of Medical Sciences College of 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