{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T16:49:03Z","timestamp":1788454143270,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T00:00:00Z","timestamp":1553126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AI080581"],"award-info":[{"award-number":["R01 AI080581"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"Summary<\/jats:title>Baseline eosinophils reside in the gastrointestinal tract; however, in several allergic disorders, excessive eosinophils accumulate in the blood as well in the tissues. Recently, we showed in\u00a0vitro<\/jats:italic> that interleukin (IL<\/jats:styled-content>)\u201018 matures and transforms IL<\/jats:styled-content>\u20105\u2010generated eosinophils into the pathogenic eosinophils that are detected in human allergic diseases. To examine the role of local induction of IL<\/jats:styled-content>\u201018 in promoting eosinophil\u2010associated intestinal disorders, we generated enterocyte IL<\/jats:styled-content>\u201018\u2010overexpressing mice using the rat intestinal fatty acid\u2010binding promoter (Fabpi) and analysed tissue IL<\/jats:styled-content>\u201018 overexpression and eosinophilia by performing real\u2010time polymerase chain reaction, Enzyme\u2010Linked Immunosorbent Assay and anti\u2010major basic protein immunostaining. Herein we show that Fabpi\u2010IL<\/jats:styled-content>\u201018 mice display highly induced IL<\/jats:styled-content>\u201018 mRNA<\/jats:styled-content> and protein in the jejunum. IL<\/jats:styled-content>\u201018 overexpression in enterocytes promotes marked increases of eosinophils in the blood and jejunum. Our analysis shows IL<\/jats:styled-content>\u201018 overexpression in the jejunum induces a specific population of CD<\/jats:styled-content>101+\u00a0<\/jats:sup>CD<\/jats:styled-content>274+<\/jats:sup> tissue eosinophils. Additionally, we observed comparable tissue eosinophilia in IL<\/jats:styled-content>\u201013\u2010deficient\u2010Fabpi\u2010IL<\/jats:styled-content>\u201018 mice, and reduced numbers of tissue eosinophils in eotaxin\u2010deficient\u2010Fabpi\u2010IL<\/jats:styled-content>\u201018 and IL<\/jats:styled-content>\u20105\u2010deficient\u2010Fabpi\u2010IL<\/jats:styled-content>\u201018 mice compared with Fabpi\u2010IL<\/jats:styled-content>\u201018 transgenic mice. Notably, jejunum eosinophilia in IL<\/jats:styled-content>\u20105\u2010deficient\u2010Fabpi\u2010IL\u2010<\/jats:styled-content>18 mice is significantly induced compared with wild\u2010type mice, which indicates the direct role of induced IL<\/jats:styled-content>\u201018 in the tissue accumulation of eosinophils and mast cells. Furthermore, we also found that overexpression of IL<\/jats:styled-content>\u201018 in the intestine promotes eosinophil\u2010associated peanut\u2010induced allergic responses in mice. Taken together, we provide direct in\u00a0vivo<\/jats:italic> evidence that induced expression of IL<\/jats:styled-content>\u201018 in the enterocytes promotes eotaxin\u20101, IL<\/jats:styled-content>\u20105 and IL<\/jats:styled-content>\u201013 independent intestinal eosinophilia, which signifies the clinical relevance of induced IL<\/jats:styled-content>\u201018 in eosinophil\u2010associated gastrointestinal disorders (EGIDs) to food allergens.<\/jats:p>","DOI":"10.1111\/imm.13051","type":"journal-article","created":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T09:50:09Z","timestamp":1550569809000},"page":"110-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Intestinal overexpression of IL<\/scp>\u201018 promotes eosinophils\u2010mediated allergic disorders"],"prefix":"10.1111","volume":"157","author":[{"given":"Alok K.","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Medicine Section\u00a0of Pulmonary Diseases Tulane Eosinophilic Disorder Center Tulane University School of Medicine New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemanth Kumar","family":"Kandikattu","sequence":"additional","affiliation":[{"name":"Department of Medicine Section\u00a0of Pulmonary Diseases Tulane Eosinophilic Disorder Center Tulane University School of Medicine New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murli","family":"Manohar","sequence":"additional","affiliation":[{"name":"Department of Medicine Section\u00a0of Pulmonary Diseases Tulane Eosinophilic Disorder Center Tulane University School of Medicine New Orleans LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshi","family":"Shukla","sequence":"additional","affiliation":[{"name":"Department of Medicine Section\u00a0of Pulmonary Diseases Tulane Eosinophilic Disorder Center Tulane University School of Medicine New 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