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Conventional enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">cELISA<\/jats:styled-content>) based on several parasitic antigens is the main method for diagnosis and indication of treatment. Dissociative <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">dELISA<\/jats:styled-content>) uses acidic treatment to free immunoglobulin G (IgG) from immune complexes, and its use revealed a significant positive fraction of suspected cases with negative serology. Looking for small molecules or haptens that block IgG antibodies, we purified by molecular exclusion chromatography, 1000\u20133000 MW molecules from promastigote soluble extract, mostly oligosaccharides comprising 6\u201313 sugar residues using <jats:styled-content style=\"fixed-case\">MALDI<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TOF<\/jats:styled-content> analysis. Glycan\u2010<jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content> complex (<jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content>) was constructed by conjugating promastigote glycans to <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content> molecules, allowing their use in the solid support in <jats:styled-content style=\"fixed-case\">cELISA<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">dELISA<\/jats:styled-content>. Sera from experimentally infected hamsters showed higher levels of blocked monomeric IgG during infection, mostly against <jats:styled-content style=\"fixed-case\">GBC<\/jats:styled-content>, which was also present in lower concentrations in the promastigote soluble extract <jats:styled-content style=\"fixed-case\">dELISA<\/jats:styled-content>. Those data show that most of the specific monomeric IgG in serum are blocked by haptens composed by glycans produced by the parasite, better detected in the high dilution of sera in the <jats:styled-content style=\"fixed-case\">dELISA<\/jats:styled-content> assays. <jats:styled-content style=\"fixed-case\">dELISA<\/jats:styled-content> is a useful technique for detecting blocked monomeric antibodies that could have difficult clearance from blood, which could result in hypergammaglobulinemia.<\/jats:p>","DOI":"10.1111\/imm.13118","type":"journal-article","created":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T03:13:36Z","timestamp":1569986016000},"page":"314-321","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High levels of serum glycans monovalent IgG immune complexes detected by dissociative <scp>ELISA<\/scp> in experimental visceral leishmaniasis"],"prefix":"10.1111","volume":"158","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-2749","authenticated-orcid":false,"given":"Camila Aparecida","family":"de Carvalho","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Fidelis","family":"Ferr\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1via Regina Novais","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"J\u00fanior","given":"Heitor Franco","family":"de Andrade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia Instituto de Medicina Tropical da Universidade de S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"World Health Organization.Leishmaniasis. 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