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The role of annexin A1 (AnxA1) in the innate immune system has focused mainly on the anti\u2010inflammatory and proresolving properties through its binding to the formyl\u2010peptide receptor 2 (FPR2)\/ALX receptor. However, studies suggesting an intracellular role of AnxA1 are emerging. In this study, we aimed to understand the role of AnxA1 for interleukin (IL)\u20101\u03b2 release in response to activators of the nucleotide\u2010binding domain leucine\u2010rich repeat (NLR) and pyrin domain containing receptor 3 (NLRP3) inflammasome. Using AnxA1 knockout mice, we observed that AnxA1 is required for IL\u20101\u03b2 release <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. These effects were due to reduction of transcriptional levels of IL\u20101\u03b2, NLRP3 and caspase\u20101, a step called NLRP3 priming. Moreover, we demonstrate that AnxA1 co\u2010localize and directly bind to NLRP3, suggesting the role of AnxA1 in inflammasome activation is independent of its anti\u2010inflammatory role via FPR2. Therefore, AnxA1 regulates NLRP3 inflammasome priming and activation in a FPR2\u2010independent manner.<\/jats:p>","DOI":"10.1111\/imm.13184","type":"journal-article","created":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T12:18:11Z","timestamp":1582892291000},"page":"78-89","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["The role of annexin A1 in the modulation of the NLRP3 inflammasome"],"prefix":"10.1111","volume":"160","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9943-8349","authenticated-orcid":false,"given":"Izabela","family":"Galv\u00e3o","sequence":"first","affiliation":[{"name":"Departamento de Morfologia Instituto de Ci\u00eancias Biol\u00f3gicas Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5740-0652","authenticated-orcid":false,"given":"Renan V. 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