{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T08:07:32Z","timestamp":1790755652052,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,7,13]],"date-time":"2020-07-13T00:00:00Z","timestamp":1594598400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["FC001159"],"award-info":[{"award-number":["FC001159"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["FC001159"],"award-info":[{"award-number":["FC001159"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010438","name":"Francis Crick Institute","doi-asserted-by":"publisher","award":["FC001159"],"award-info":[{"award-number":["FC001159"]}],"id":[{"id":"10.13039\/100010438","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000289","name":"Cancer Research UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000289","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The implication of complement in multiple diseases over the last 20\u00a0years has fuelled interest in developing anti\u2010complement drugs. To date, the focus has been on C5; blocking cleavage of C5 prevents formation of two pro\u2010inflammatory activities, C5a anaphylatoxin and membrane attack complex. The concept of C5 blockade to inhibit inflammation dates back 30\u00a0years to the description of BB5.1, an anti\u2010C5 blocking monoclonal antibody raised in C5\u2010deficient mice. This antibody proved an invaluable tool to demonstrate complement involvement in mouse disease models and catalysed enthusiasm for anti\u2010complement drug development, culminating in the anti\u2010human C5 monoclonal antibody eculizumab, the most successful anti\u2010complement drug to date, already in clinical use for several rare diseases. Despite its key role in providing proof\u2010of\u2010concept for C5 blockade, the mechanism of BB5.1 inhibition remains poorly understood. Here, we characterized BB5.1 cross\u2010species inhibition, C5 binding affinity and chain specificity. BB5.1 efficiently inhibited C5 in mouse serum but not in human or other rodent sera; it prevented C5 cleavage and C5a generation. BB5.1 bound the C5\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010chain with high affinity and slow off\u2010rate. BB5.1 complementarity\u2010determining regions were obtained and docking algorithms were used to predict the likely binding interface on mouse C5.\n                  <\/jats:p>","DOI":"10.1111\/imm.13228","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T17:59:45Z","timestamp":1592416785000},"page":"103-113","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterizing the original anti\u2010C5 function\u2010blocking antibody, BB5.1, for species specificity, mode of action and interactions with C5"],"prefix":"10.1111","volume":"161","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2230-3550","authenticated-orcid":false,"given":"Wioleta M.","family":"Zelek","sequence":"first","affiliation":[{"name":"Systems Immunity University Research Institute School of Medicine Cardiff University Cardiff UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgina 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