{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:28:55Z","timestamp":1762900135896,"version":"3.40.5"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002570","name":"American Association of Immunologists","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002570","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI135537"],"award-info":[{"award-number":["AI135537"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM104317\u20105"],"award-info":[{"award-number":["P20GM104317\u20105"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>There is growing interest in understanding antibody (Ab) function beyond neutralization. The non\u2010structural protein 1 (NS1) of Zika virus (ZIKV) is an attractive candidate for an effective vaccine as Abs against NS1, unlike the envelope or premembrane, do not carry the risk of mediating antibody\u2010dependent enhancement. Our aim was to evaluate whether ZIKV NS1 Abs elicited following natural infection in humans can mediate antibody\u2010dependent cellular cytotoxicity (ADCC). We evaluated the isotype specificity of ZIKV\u2010specific Abs in immune sera and supernatants from stimulated immune PBMC and found that Abs against ZIKV NS1 and virus\u2010like particles were predominantly of the IgG1 isotype. Using a recently developed FluoroSpot assay, we found robust frequencies of NS1\u2010specific Ab\u2010secreting cells in PBMC of individuals who were naturally infected with ZIKV. We developed assays to measure both natural killer cell activation by flow cytometry and target cell lysis of ZIKV NS1\u2010expressing cells using an image cytometry assay in the presence of ZIKV NS1 Abs. Our data indicate efficient opsonization of ZIKV NS1\u2010expressing CEM\u2010NK<jats:sup>R<\/jats:sup> cell lines using ZIKV\u2010immune but not ZIKV\u2010na\u00efve sera, a prerequisite of ADCC. Furthermore, sera from immune donors were able to induce both NK cell degranulation and lysis of ZIKV NS1 CEM\u2010NK<jats:sup>R<\/jats:sup> cells in vitro. Our data suggest that ADCC is a possible mechanism for ZIKV NS1 Abs to eliminate virally infected target cells.<\/jats:p>","DOI":"10.1111\/imm.13380","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T08:35:48Z","timestamp":1622450148000},"page":"386-397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Non\u2010structural protein 1\u2010specific antibodies directed against Zika virus in humans mediate antibody\u2010dependent cellular cytotoxicity"],"prefix":"10.1111","volume":"164","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4622-5427","authenticated-orcid":false,"given":"Luis A.","family":"Sanchez Vargas","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology Institute for Immunology and Informatics University of Rhode Island Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Awadalkareem","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Institute for Immunology and Informatics University of Rhode Island Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Masterson","sequence":"additional","affiliation":[{"name":"Viral Diseases Branch Walter Reed Army Institute of Research Silver Spring MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madison","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Institute for Immunology and Informatics University of Rhode Island Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe L.","family":"Lyski","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology Oregon Health &amp; Science University Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly A.","family":"Dowd","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Diseases NIAID, NIH Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theodore C.","family":"Pierson","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Diseases NIAID, NIH Bethesda MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William B.","family":"Messer","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology and Immunology Oregon Health &amp; Science University Portland OR USA"},{"name":"Division of Infectious Diseases Department of Medicine Oregon Health &amp; Science University Portland OR USA"},{"name":"OHSU\u2010PSU School of Public Health Program in Epidemiology Oregon Health &amp; Science University Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey R.","family":"Currier","sequence":"additional","affiliation":[{"name":"Viral Diseases Branch Walter Reed Army Institute of Research Silver Spring MD USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6752-009X","authenticated-orcid":false,"given":"Anuja","family":"Mathew","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology Institute for Immunology and Informatics University of Rhode Island Providence RI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00252-16"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00072-15"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007640"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12681"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2020.08.006"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan6836"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb6143"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18938"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3515"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00562-6"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00651-6"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.8575"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30902-3"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.01.005"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01651"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006673"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005738"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz331"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1015"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00879-07"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaa3787"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19096-y"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03179-20"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb9425"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc0476"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz081"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/imcb.12312"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2020.112900"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008006"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.07.049"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800892"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7050035"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.09.002"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.12.002"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines7030103"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv181"},{"issue":"5","key":"e_1_2_11_38_1","first-page":"818","article-title":"Human seasonal influenza A viruses induce H7N9\u2010cross\u2010reactive antibody\u2010dependent cellular cytotoxicity (ADCC) antibodies that are directed towards the nucleoprotein","volume":"215","author":"Jegaskanda S","year":"2016","journal-title":"J Infect Dis"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1086\/512860"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900985"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aai8128"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07008-0"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.13380","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imm.13380","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.13380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T08:11:00Z","timestamp":1693296660000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.13380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,14]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1111\/imm.13380"],"URL":"https:\/\/doi.org\/10.1111\/imm.13380","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"type":"print","value":"0019-2805"},{"type":"electronic","value":"1365-2567"}],"subject":[],"published":{"date-parts":[[2021,6,14]]},"assertion":[{"value":"2021-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-05-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}