{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:52:29Z","timestamp":1780469549821,"version":"3.54.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T00:00:00Z","timestamp":1669075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001542","name":"Children's Cancer and Blood Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001542","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005190","name":"Melanoma Research Alliance","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009784","name":"Starr Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009784","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Metastatic disease is the major cause of death from cancer. From the primary tumour, cells remotely prepare the environment of the future metastatic sites by secreted factors and extracellular vesicles. During this process, known as pre\u2010metastatic niche formation, immune cells play a crucial role. Mast cells are haematopoietic bone marrow\u2010derived innate immune cells whose function in lung immune response to invading tumours remains to be defined. We found reduced melanoma lung metastasis in mast cell\u2010deficient mouse models (Wsh and MCTP5\u2010Cre\u2010RDTR), supporting a pro\u2010metastatic role for mast cells in vivo. However, due to evidence pointing to their antitumorigenic role, we studied the impact of mast cells in melanoma cell function in vitro. Surprisingly, in vitro co\u2010culture of bone\u2010marrow\u2010derived mast cells with melanoma cells showed that they have an intrinsic anti\u2010metastatic activity. Mass spectrometry analysis of melanoma\u2010mast cell co\u2010cultures secretome showed that HMGA1 secretion by melanoma cells was significantly impaired. Consistently, HMGA1 knockdown in B16\u2010F10 cells reduced their metastatic capacity in vivo. Importantly, analysis of HMGA1 expression in human melanoma tumours showed that metastatic tumours with high HMGA1 expression are associated with reduced overall and disease\u2010free survival. Moreover, we show that HMGA1 is reduced in the nuclei and enriched in the cytoplasm of melanoma metastatic lesions when compared to primary tumours. These data suggest that high HMGA1 expression and secretion from melanoma cells promote metastatic behaviour. Targeting HMGA1 expression intrinsically or extrinsically by mast cells actions reduce melanoma metastasis. Our results pave the way to the use of HMGA1 as anti\u2010metastatic target in melanoma as previously suggested in other cancer types.<\/jats:p>","DOI":"10.1111\/imm.13604","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T01:58:13Z","timestamp":1668045493000},"page":"362-373","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Mast cells impair melanoma cell homing and metastasis by inhibiting\n                    <scp>HMGA1<\/scp>\n                    secretion"],"prefix":"10.1111","volume":"168","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0700-3891","authenticated-orcid":false,"given":"Alberto","family":"Benito\u2010Martin","sequence":"first","affiliation":[{"name":"Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology Drukier Institute for Children's Health and the Meyer Cancer Center, Weill Cornell Medical College  New York New York USA"},{"name":"Universidad Alfonso X El Sabio, Facultad de Medicina Unidad de Investigaci\u00f3n Biom\u00e9dica  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7592-4430","authenticated-orcid":false,"given":"Laura","family":"Nogu\u00e9s","sequence":"additional","affiliation":[{"name":"Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology Drukier Institute for Children's Health and the Meyer Cancer Center, Weill Cornell Medical College  New York New York USA"},{"name":"Microenvironment and Metastasis Laboratory, Molecular Oncology Program Spanish National Cancer Research Center (CNIO)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Hergueta\u2010Redondo","sequence":"additional","affiliation":[{"name":"Microenvironment and Metastasis Laboratory, Molecular Oncology Program Spanish National Cancer Research Center (CNIO)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0851-129X","authenticated-orcid":false,"given":"Elena","family":"Castellano\u2010Sanz","sequence":"additional","affiliation":[{"name":"Microenvironment and Metastasis Laboratory, Molecular Oncology Program Spanish National Cancer Research Center (CNIO)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Garvin","sequence":"additional","affiliation":[{"name":"Microenvironment and Metastasis Laboratory, Molecular Oncology Program Spanish National Cancer Research Center (CNIO)  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4180-5369","authenticated-orcid":false,"given":"Michele","family":"Cioffi","sequence":"additional","affiliation":[{"name":"Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology Drukier Institute for Children's Health and the Meyer Cancer Center, Weill Cornell Medical College  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1554-0344","authenticated-orcid":false,"given":"Paloma","family":"Sola\u2010Castrillo","sequence":"additional","affiliation":[{"name":"Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology Drukier Institute for Children's Health and the Meyer Cancer Center, Weill Cornell Medical College  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0946-0671","authenticated-orcid":false,"given":"Weston","family":"Buehring","sequence":"additional","affiliation":[{"name":"Children's Cancer and Blood Foundation Laboratories, Departments of Pediatrics, and Cell and Developmental Biology Drukier Institute for Children's Health and the Meyer 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