{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:51:19Z","timestamp":1790268679913,"version":"4.1.0"},"reference-count":104,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T00:00:00Z","timestamp":1680048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81770035"],"award-info":[{"award-number":["81770035"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    IL\u201017A\u2010producing group 3 innate lymphoid cells (ILC3s) have been found to participate in the development of various phenotypes of asthma, however, little is known about how ILC3s mediate neutrophilic airway inflammation. Elevated IL\u20101\u03b2 has been reported in neutrophilic asthma (NA) and IL\u20101\u03b2 receptor is highly expressed on lung ILC3s. Therefore, we hypothesize that IL\u20101\u03b2 aggravates neutrophilic airway inflammation via provoking IL\u201017A\u2010producing ILC3s. We sought to determine the pathological roles of the IL\u20101\u03b2\u2010ILC3\u2010IL\u201017A axis in neutrophilic airway inflammation. Lung ILC subsets were measured in eosinophilic asthma (ovalbumin [OVA]\/Alum) and NA (OVA\/lipopolysaccharides [LPS]) murine models. Rag2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    (lacking adaptive immunity), RORc\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    (lacking transcription factor ROR\u03b3t), Rag2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    RORc\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    (lacking adaptive immunity and ILC3s), and ILCs depletion mice were used to verify the roles of ILC3s in neutrophilic airway inflammation by measurement of CXCL\u20101, IL\u201017A, IL\u201022 and neutrophil counts in bronchoalveolar lavage fluid (BALF), detection of Muc5ac in lung tissues, and quantification of IL\u201017A\u2010producing ILC3s after treatment of anti\u2010IL\u201017A or recombinant IL\u20101\u03b2 (rIL\u20101\u03b2) and its monoclonal antibody. NLRP3, Caspase 1 and their induction of IL\u20101\u03b2 were detected in lung tissues of OVA\/LPS\u2010induced mice. The OVA\/LPS model was characterized by an enrichment of airway neutrophilia, lung ROR\u03b3t\n                    <jats:sup>+<\/jats:sup>\n                    ILC3s and Th17 cytokines (IL\u201017A and IL\u201022) and neutrophilic chemokine C\u2010X\u2010C motif (chemokine) ligand 1 (CXCL\u20101), compared to the phenotypic features of airway eosinophilia, GATA3\n                    <jats:sup>+<\/jats:sup>\n                    ILC2s and type\u20102 cytokines in OVA\/Alum model. The concentration of CXCL\u20101 and neutrophil counts in BALF were decreased by anti\u2010IL\u201017A. ROR\u03b3t deficiency led to a decrease in IL\u201017A and CXCL\u20101 levels and neutrophil counts in BALF. ILC depletion in Rag2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice ameliorated OVA\/LPS\u2010induced IL\u201017A, IL\u201022, CXCL\u20101 and airway neutrophil counts. IL\u201017A\u2010producing ILCs and BALF neutrophil counts were significantly lower in Rag2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    RORc\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice than those in Rag2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. IL\u20101\u03b2 was highly expressed in BALF and bronchial epithelial cells (BECs) in OVA\/LPS model, and administration of rIL\u20101\u03b2 substantially aggravated airway inflammation and promoted upregulation of ROR\u03b3t\n                    <jats:sup>+<\/jats:sup>\n                    and IL\u201017A\u2010producing lung ILC3s, which were reversed by anti\u2010IL\u20101\u03b2. NLRP3 and Caspase 1 expressions were enhanced by OVA\/LPS, and their inhibitors abolished the OVA\/LPS\u2010induced IL\u20101\u03b2 in BECs. ILC3s play a pathogenic role in the pathogenesis of NA, which is triggered by IL\u20101\u03b2 via promoting IL\u201017A production of lung ILC3s.\n                  <\/jats:p>","DOI":"10.1111\/imm.13644","type":"journal-article","created":{"date-parts":[[2023,3,29]],"date-time":"2023-03-29T11:19:22Z","timestamp":1680088762000},"page":"16-32","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["IL\u20101\u03b2 promotes IL\u201017A production of ILC3s to aggravate neutrophilic airway inflammation in mice"],"prefix":"10.1111","volume":"176","author":[{"given":"Dan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Geriatrics and National Clinical Research Center for Geriatrics, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi'na","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yang","sequence":"additional","affiliation":[{"name":"Clinical Trial Center, National Medical Products Administration Key Laboratory for Clinical Research and Evaluation of Innovative Drugs, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixiu","family":"He","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingxuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanlan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Luo","sequence":"additional","affiliation":[{"name":"Respiratory Medicine Unit and NIHR Oxford Biomedical Research Centre University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8823-480X","authenticated-orcid":false,"given":"Chuntao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine, West China School of Medicine and West China Hospital Sichuan University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201504-0763PP"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2678"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200903-0392OC"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2466-13-11"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2006.00784.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69290-8"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.10.011"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-018-8712-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3786"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-31491-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14189"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.07.017"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-075053"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.02.015"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12353"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.09.037"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.05.042"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.03.008"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aan8546"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.05.012"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.03.015"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.664218"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.542818"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0000000000000497"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15866"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.930862"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.688879"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.10.040"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.586471"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.09.011"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3423"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.03.019"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-019-1015-0"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00144"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.06.037"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.07.046"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-019-0361-9"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2019.101333"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201609-1830OC"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100500"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00105013"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13619"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12400"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-215986"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00528-2020"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2019.08.2192"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001713"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.8.5375"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2010.02352.x"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.2147\/JAA.S121092"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.5.3540"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.12.7207"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.000323"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1082-x"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201410-1782OC"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.017"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1065544"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00027.2009"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3489"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.02.016"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3109\/10715760903362576"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.03.044"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100645"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00405-2015"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.08.041"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2005590118"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.04.038"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.13415"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.01.003"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12793"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2014-206067"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0282-9"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201545598"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202386"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.918458"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.946905"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.07.005"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3078"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam5809"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3443"},{"key":"e_1_2_10_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2013.08.002"},{"key":"e_1_2_10_83_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2684"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aab3142"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20092054"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12907"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.05.038"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.4832"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.132836"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201212-2318OC"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.10.038"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071507"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1300039"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2045"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202924"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05141-x"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2018.03.007"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.849155"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00092"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1276-2"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2013.33"},{"key":"e_1_2_10_102_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.08.061"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.11.033"},{"key":"e_1_2_10_104_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.02.045"},{"key":"e_1_2_10_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.09.035"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.13644","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T02:47:48Z","timestamp":1754275668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.13644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,29]]},"references-count":104,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/imm.13644"],"URL":"https:\/\/doi.org\/10.1111\/imm.13644","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,29]]},"assertion":[{"value":"2022-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}