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Conversely, nutritional imbalance and impaired glucose metabolism are associated with chronic inflammation, aberrant immunity and anomalous leukocyte trafficking. Despite being exposed to periodic alterations in blood insulin levels upon fasting and feeding, studies exploring the physiological effects of these hormonal changes on quiescent immune cell function and trafficking are scanty. Here, we report that oral glucose load in mice and healthy men enhances the adherence of circulating peripheral blood mononuclear cells (PBMCs) and lymphocytes to fibronectin. Adherence to fibronectin is also observed upon regular intake of breakfast following overnight fasting in healthy subjects. This glucose load\u2010induced phenomenon is abrogated in streptozotocin\u2010injected mice that lack insulin. Intra\u2010vital microscopy in mice demonstrated that oral glucose feeding enhances the homing of PBMCs to injured blood vessels in vivo. Furthermore, employing flow cytometry, Western blotting and adhesion assays for PBMCs and Jurkat\u2010T cells, we elucidate that insulin enhances fibronectin adherence of quiescent lymphocytes through non\u2010canonical signalling involving insulin\u2010like growth factor\u20101 receptor (IGF\u20101R) autophosphorylation, phospholipase C gamma\u20101 (PLC\u03b3\u20101) Tyr<jats:sup>783<\/jats:sup> phosphorylation and inside\u2010out activation of \u03b2\u2010integrins respectively. Our findings uncover the physiological relevance of post\u2010prandial insulin spikes in regulating the adherence and trafficking of circulating quiescent T\u2010cells through fibronectin\u2013integrin interaction.<\/jats:p>","DOI":"10.1111\/imm.13652","type":"journal-article","created":{"date-parts":[[2023,4,26]],"date-time":"2023-04-26T06:41:29Z","timestamp":1682491289000},"page":"60-82","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Feeding enhances fibronectin adherence of quiescent lymphocytes through non\u2010canonical insulin signalling"],"prefix":"10.1111","volume":"170","author":[{"given":"Abhiram Charan Tej","family":"Mallu","sequence":"first","affiliation":[{"name":"Centre of Excellence (CoE) in Molecular Medicine, Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences Indian Institute of Technology Madras  Chennai 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