{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:19:18Z","timestamp":1747185558623,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:00:00Z","timestamp":1699833600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In a Japanese chemical factory, lung diseases such as pneumoconiosis have been reported among workers handling cross\u2010linked water\u2010soluble acrylic acid polymers (CWAAP). Our previous study reported that a single intratracheal administration of CWAAP induces acute inflammation and fibrosis. In this study, we investigated the effects of multiple intratracheal administrations of CWAAP on inflammatory responses and pulmonary fibrosis along with inducible bronchus\u2010associated lymphoid tissues (iBALT) formation, which is involved in allergic inflammation. Male F344 rats (190\u2013200\u2009g) received single or multiple intratracheal administrations of phosphate\u2010buffered saline (PBS) or CWAAP. To assess inflammatory responses and pulmonary fibrosis, immunohistochemical and histological staining was performed. CD68, CD163, CD169, TGF\u2010\u03b2, and collagen I positive cells\/areas in the lungs of the CWAAP\u2010group rats were significantly increased than those in the PBS group. Furthermore, the number of iBALT structures, CD4\u2009+\u2009T cells, along with CD19, PAX5, IL\u20104, GATA\u20103, T\u2010bet, and IgE\u2010positive cells in the terminal bronchioles and blood vessels of the lungs were significantly increased in the CWAAP group. Moreover, pulmonary fibrosis, iBALT formation, and levels of specific IgG were significantly increased in rats who received multiple intratracheal administrations of CWAAP compared to those with single intratracheal administration. Multiple intratracheal administrations of CWAAP potentiated the classical fibrotic pathway (M2 macrophage\u2010TGF\u2010\u03b2\u2010collagen I) more potently than single intratracheal administration. Furthermore, it was possible that iBALT was formed around terminal bronchioles and blood vessels and the number of immune cells was increased, resulting in enhanced allergic inflammation and pulmonary fibrosis.<\/jats:p>","DOI":"10.1111\/imm.13715","type":"journal-article","created":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T06:39:39Z","timestamp":1699943979000},"page":"250-261","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intratracheal administration of cross\u2010linked water\u2010soluble acrylic acid polymer is associated with inducible bronchi\u2010related lymphoid tissue formation and allergic inflammation"],"prefix":"10.1111","volume":"171","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2537-2586","authenticated-orcid":false,"given":"Takamasa","family":"Kido","sequence":"first","affiliation":[{"name":"Department of Public Health and Environmental Medicine The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiemi","family":"Sugaya","sequence":"additional","affiliation":[{"name":"Department of Public Health and Environmental Medicine The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Hano","sequence":"additional","affiliation":[{"name":"The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5241-5238","authenticated-orcid":false,"given":"Hiroyuki","family":"Yanagisawa","sequence":"additional","affiliation":[{"name":"The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Machi","family":"Suka","sequence":"additional","affiliation":[{"name":"Department of Public Health and Environmental Medicine The Jikei University School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tox.2020.152647"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12989-022-00468-9"},{"key":"e_1_2_12_4_1","unstructured":"Ministry of Health Labour and Welfare.Alert notice about incidence of lung diseases among workers handling cross\u2010linked water\u2010soluble acrylic acid polymer in a chemical factory.2017(in Japanese). Available from:https:\/\/www.mhlw.go.jp\/stf\/houdou\/0000163568.html. Accessed 7 July 2020a."},{"key":"e_1_2_12_5_1","unstructured":"Ministry of Health Labour and Welfare.Committee report on lung diseases among workers handling cross\u2010linked water\u2010soluble acrylic acid polymer in a chemical factory.2019(in Japanese). Available from:https:\/\/www.mhlw.go.jp\/stf\/shingi\/other-roudou_128880_00004.html. 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