{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:18:39Z","timestamp":1787163519454,"version":"build-2736575974"},"reference-count":84,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Latent human cytomegalovirus (hCMV) infection can pose a serious threat of reactivation and disease occurrence in immune\u2010compromised individuals. Although T cells are at the core of the protective immune response to hCMV infection, a detailed characterization of different T cell subsets involved in hCMV immunity is lacking. Here, in an unbiased manner, we characterized over 8000 hCMV\u2010reactive peripheral memory T cells isolated from seropositive human donors, at a single\u2010cell resolution by analysing their single\u2010cell transcriptomes paired with the T cell antigen receptor (TCR) repertoires. The hCMV\u2010reactive T cells were highly heterogeneous and consisted of different developmental and functional memory T cell subsets such as, long\u2010term memory precursors and effectors, T helper\u201017, T regulatory cells (T\n                    <jats:sub>REGs<\/jats:sub>\n                    ) and cytotoxic T lymphocytes (CTLs) of both CD4 and CD8 origin. The hCMV\u2010specific T\n                    <jats:sub>REGs<\/jats:sub>\n                    , in addition to being enriched for molecules known for their suppressive functions, showed enrichment for the interferon response signature gene sets. The hCMV\u2010specific CTLs were of two types, the pre\u2010effector\u2010 and effector\u2010like. The co\u2010clustering of hCMV\u2010specific CD4\u2010CTLs and CD8\u2010CTLs in both pre\u2010effector as well as effector clusters suggest shared transcriptomic signatures between them. The huge TCR clonal expansion of cytotoxic clusters suggests a dominant role in the protective immune response to CMV. The study uncovers the heterogeneity in the hCMV\u2010specific memory T cells revealing many functional subsets with potential implications in better understanding of hCMV\u2010specific T cell immunity. The data presented can serve as a knowledge base for designing vaccines and therapeutics.\n                  <\/jats:p>","DOI":"10.1111\/imm.13783","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T06:00:57Z","timestamp":1710828057000},"page":"420-439","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Single\u2010cell transcriptomic and\n                    <scp>T cell antigen receptor<\/scp>\n                    analysis of human cytomegalovirus (\n                    <scp>hCMV<\/scp>\n                    )\u2010specific memory T cells reveals effectors and pre\u2010effectors of\n                    <scp>CD8<\/scp>\n                    <sup>+<\/sup>\n                    \u2010 and\n                    <scp>CD4<\/scp>\n                    <sup>+<\/sup>\n                    \u2010cytotoxic T cells"],"prefix":"10.1111","volume":"172","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1971-5682","authenticated-orcid":false,"given":"Raunak","family":"Kar","sequence":"first","affiliation":[{"name":"Immunogenomics Lab National Institute of Immunology  New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somdeb","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"Immunogenomics Lab National Institute of Immunology  New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine and Blood Bank Vardhman Mahavir Medical College and Safdarjung Hospital  New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Immunogenomics Lab National Institute of Immunology  New Delhi Delhi 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