{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:26:52Z","timestamp":1787318812404,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T00:00:00Z","timestamp":1729382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270562"],"award-info":[{"award-number":["82270562"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200591"],"award-info":[{"award-number":["82200591"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302800"],"award-info":[{"award-number":["82302800"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Regulator of G\u2010protein signalling (RGS) 10 plays critical roles in several immune related diseases. However, whether RGS10 is involved in colonic inflammation of ulcerative colitis (UC) is still obscure. This study aimed to investigate the role of RGS10 in UC. In this study, RGS10 expression was examined by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR), western blotting, immunohistochemistry, and immunofluorescent analysis. Single\u2010cell RNA sequencing of intestinal mucosa was performed to identify key immune cells with differentially expressed RGS10. RGS10 knockout mice were generated and established dextran sulphate sodium (DSS)\u2010induced colitis. Expression of inflammatory cytokines on mRNA and protein levels was detected by qRT\u2010PCR, enzyme\u2010linked immunosorbent assay, and flow cytometry. We found that RGS10 expression was significantly elevated in UC patients, especially in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, compared with healthy subjects. Intriguingly, RGS10 deficiency markedly alleviated DSS\u2010induced colitis and decreased the proportion of Th1 and Th17 cells in lamina propria mononuclear cells (LPMCs), peripheral blood (PB), spleens, and mesenteric lymph nodes (mLNs). Mechanistically, RGS10 deficiency blocked the differentiation of Th1 and Th17 cells by inhibiting the phosphorylation of signal transducer and activator of transcription (STAT) 1 and STAT3. The co\u2010immunoprecipitation analysis further showed that RGS10 could interact with protein tyrosine phosphatase non\u2010receptor type 2 (PTPN2), and further regulated Th1 and Th17 cells differentiation of CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. In conclusion, RGS10 deficiency alleviated intestinal mucosal inflammation through inhibition of Th1\/Th17 cell\u2010mediated immune responses via interaction with PTPN2 in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells. Therefore, targeting RGS10 may represent a novel therapeutic approach for UC treatment.\n                  <\/jats:p>","DOI":"10.1111\/imm.13869","type":"journal-article","created":{"date-parts":[[2024,10,20]],"date-time":"2024-10-20T22:15:00Z","timestamp":1729462500000},"page":"139-152","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>RGS10<\/scp>\n                    Deficiency Alleviated Intestinal Mucosal Inflammation Through Suppression of Th1\/Th17 Cell Immune Responses in Ulcerative Colitis"],"prefix":"10.1111","volume":"174","author":[{"given":"Yonghong","family":"Yang","sequence":"first","affiliation":[{"name":"Medical Research Center Affiliated Hospital of Jining Medical University  Jining Shandong 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