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Alongside enhancing diagnostic capabilities and bolstering public health initiatives, developing a safe and effective vaccine represents a promising strategy to tackle this health challenge. In this paper, we employed an array of bioinformatics tools to engineer a subunit vaccine that exhibits high immunogenicity against <jats:styled-content style=\"fixed-case\"><jats:italic>S. anginosus<\/jats:italic><\/jats:styled-content>. After constructing the multi\u2010epitope vaccine, we subsequently predicted its secondary and tertiary protein structures. After refining and validating the modelled structure, we utilised advanced computational approaches, including molecular docking and dynamic simulations, to evaluate the binding affinity, compatibility, and stability of the vaccine\u2013adjuvant complexes. Eventually, in silico cloning was conducted to optimise protein expression and production. The multi\u2010epitope subunit vaccine we developed showed properties in antigenicity and immunity theoretically. The computational study revealed that this vaccine demonstrates significant efficacy against <jats:styled-content style=\"fixed-case\"><jats:italic>S. anginosus<\/jats:italic><\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/imm.13936","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T23:09:17Z","timestamp":1745449757000},"page":"339-358","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a Novel Multi\u2010Epitope Vaccine Against <scp><i>Streptococcus anginosus<\/i><\/scp> Infection via Reverse Vaccinology Approach"],"prefix":"10.1111","volume":"175","author":[{"given":"Linglan","family":"Xu","sequence":"first","affiliation":[{"name":"Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Institute of Pathogenic Biology, Hengyang Medical School University of South China  Hengyang Hunan China"},{"name":"Department of Clinical Laboratory Affiliated Hengyang Hospital of Hunan Normal University &amp; 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