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We have developed a multiplex immunofluorescence imaging pipeline that allows the analysis of human follicular adaptive and innate immune cell subsets. Our data revealed the in\u00a0situ phenotypic heterogeneity and differential localisation of follicular helper CD4 T (T<jats:sub>FH<\/jats:sub>) cell subsets across follicular areas in tonsils and reactive lymph nodes (LNs). Cell clustering analysis identified specific T<jats:sub>FH<\/jats:sub> subsets with differential prevalence between tonsils and LNs. Further, a multiplex RNAscope\/protein imaging assay revealed the functional heterogeneity of T<jats:sub>FH<\/jats:sub> cells. No significant differences in follicular innate immune cell densities were found between tonsils and LNs. In conclusion, we present a combinatory experimental approach that provides a comprehensive analysis of human follicular and\/or germinal centre immune dynamics and could be used to further understand the pathogenesis of diseases such as HIV and lymphomas.<\/jats:p>","DOI":"10.1111\/imm.13955","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T06:34:57Z","timestamp":1748327697000},"page":"87-104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Delineation of the Human Germinal Centre Immune Landscape Using Multiplex Imaging Analysis"],"prefix":"10.1111","volume":"176","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9398-3143","authenticated-orcid":false,"given":"Spiros","family":"Georgakis","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Institute of Pathology, Lausanne University Hospital and Lausanne University  Lausanne 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