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When expressed on the cell surface, ENO1 binds plasminogen (PLG) and promotes cell migration by facilitating plasmin (PLM)\u2010mediated extracellular matrix degradation. Here, we observed that inflammatory stimulation significantly upregulated ENO1 expression on the neutrophil surface, both in\u00a0vitro and in\u00a0vivo. An anti\u2010ENO1 monoclonal antibody (mAb), 7E5, which blocks the ENO1\u2010PLG interaction, effectively suppressed neutrophil invasion in\u00a0vitro. In mouse models of acute inflammation, including lipopolysaccharide (LPS)\u2010induced lung injury and necrotic cell challenge, 7E5 treatment markedly reduced neutrophil recruitment and neutrophil extracellular trap (NET) formation. Similarly, the PLG inhibitor tranexamic acid (TXA) attenuated neutrophil recruitment, confirming the critical role of the PLG\/PLM system in neutrophil migration. These findings highlight ENO1 as a key regulator of inflammation and neutrophil infiltration. Targeting ENO1 with antibodies could be a promising strategy to mitigate tissue damage caused by excessive neutrophilic inflammation.<\/jats:p>","DOI":"10.1111\/imm.70034","type":"journal-article","created":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T07:18:45Z","timestamp":1757402325000},"page":"137-148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Enolase\u20101 Is a Key Regulator of Neutrophil Recruitment During Acute Inflammation"],"prefix":"10.1111","volume":"177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6463-005X","authenticated-orcid":false,"given":"Hsueh\u2010Yen","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Biochemical Science and Technology National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping\u2010Hsiang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010Wei","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Wen","family":"Chang","sequence":"additional","affiliation":[{"name":"Graduate Institute of Molecular and Comparative Pathobiology, School of Veterinary Medicine National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nai\u2010Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Jing","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Biochemical Science and Technology National Taiwan University  Taipei Taiwan, ROC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ta\u2010Tung","family":"Yuan","sequence":"additional","affiliation":[{"name":"HuniLife Biotechnology Inc.  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