{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T06:01:35Z","timestamp":1783144895200,"version":"3.54.6"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T00:00:00Z","timestamp":1759363200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Laryngeal cancer (LC) is a condition characterised by cancerous cell development in laryngeal tissues. This article focuses on the mechanism of tumour\u2010associated macrophage\u2010derived exosomes (TAM\u2010Exos) in LC chemosensitivity by mediating Wilms' tumour 1\u2010associating protein (WTAP)\/glucose transporter\u20101 (GLUT\u20101). Human peripheral blood mononuclear cells (PBMCs) were cultured in\u00a0vitro. Macrophage colony\u2010stimulating factor (M\u2010CSF) was used to induce the differentiation of PBMCs into human peripheral blood macrophages (M0), which were further induced into tumour\u2010associated macrophages\u2010educated macrophages (TAMEMs\n                    <jats:sup>C\u2010h<\/jats:sup>\n                    ) by 20% human recombinant protein cocktail and transfected with sh\u2010WTAP. TU686 cells were treated with TAMEMs\n                    <jats:sup>C\u2010h<\/jats:sup>\n                    derived Exos, cisplatin (DDP), 2\u2010deoxyglucose, and small\u2010interfering\u2010GLUT\u20101, with TAMEMs\n                    <jats:sup>C\u2010h<\/jats:sup>\n                    morphology and phenotype assessed. Finally, nude mice were treated with DDP and Exos for in\u00a0vivo verification. TAMEMs\n                    <jats:sup>C\u2010h<\/jats:sup>\n                    \u2010derived Exos promoted aerobic glycolysis and reduced LC cell sensitivity to DDP. TAMEMs\n                    <jats:sup>C\u2010h<\/jats:sup>\n                    \u2010derived Exos increased glucose uptake, lactic acid, lactate dehydrogenase, and adenosine triphosphate levels, facilitated aerobic glycolysis, viability, and invasion, and decreased apoptotic rate by mediating WTAP, thus reducing DDP sensitivity in TU686 cells. But the effect of TAM\u2010Exos was reversed in response to aerobic glycolysis suppression or GLUT\u20101 knockdown. TAM\u2010Exos carrying WTAP increased GLUT\u20101 N6\u2010methyladenosine (m6A) modification and GLUT\u20101 messenger RNA (mRNA) stability, hence boosting GLUT\u20101 expression. TAM\u2010Exos boosted aerobic glycolysis by mediating WTAP\/GLUT\u20101, thus decreasing DDP sensitivity and enhancing tumour growth. TAM\u2010Exos promoted the m6A modification of GLUT\u20101 through WTAP, and improved GLUT\u20101 mRNA stability and expression, thereby promoting aerobic glycolysis and reducing the chemo\u2010sensitivity of LC.\n                  <\/jats:p>","DOI":"10.1111\/imm.70039","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T01:04:24Z","timestamp":1759453464000},"page":"270-285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mechanism of Tumour\u2010Associated Macrophage\u2010Derived Exosomes in Chemo\u2010Sensitivity of Laryngeal Cancer by Regulating Aerobic Glycolysis via\n                    <scp>WTAP<\/scp>\n                    \/\n                    <scp>GLUT<\/scp>\n                    \u20101"],"prefix":"10.1111","volume":"177","author":[{"given":"Xingmei","family":"Luo","sequence":"first","affiliation":[{"name":"The Otorhinolaryngology\u2010Head and Neck Surgery Department 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