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This study explores whether Tui Na acupressure, a traditional Chinese therapy, can restore Treg immunosuppressive function through the FoxP3\/mTORC1 signalling pathway to mitigate ALS pathology. In SOD1G93A ALS mice, Tui Na was applied at the Shenshu acupoint, with motor and cognitive functions assessed via rotarod, tail suspension, novel object recognition, and Y\u2010maze tests. Multi\u2010omics (transcriptomics, proteomics), flow cytometry, ELISA, and Western blot analysed Treg proportions, cytokine profiles, and pathway activation. In\u00a0vitro assays evaluated Treg proliferation and immunosuppression. Tui Na significantly enhanced motor and cognitive performance, increased Treg proportions in spleen, lymph nodes, and blood, and elevated anti\u2010inflammatory cytokines (IL\u201010, TGF\u2010\u03b2) while reducing pro\u2010inflammatory markers (IL\u20106, TNF\u2010\u03b1). Transcriptomic and proteomic analyses revealed upregulated FoxP3, Mtor, and Raptor, with enhanced Treg proliferation and immunosuppression confirmed in\u00a0vitro. Pathway inhibitors (GSK126, rapamycin) reversed these effects, confirming FoxP3\/mTORC1 dependency. Tui Na also reduced apoptosis and oxidative stress, supporting immune regulation. These findings highlight Tui Na's potential to restore Treg\u2010mediated immune balance in ALS, offering a non\u2010pharmacological therapeutic strategy. This study provides novel immunological insights into Tui Na's mechanisms, advocating its clinical evaluation for ALS and related immune\u2010driven disorders.<\/jats:p>","DOI":"10.1111\/imm.70052","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T15:50:52Z","timestamp":1762444252000},"page":"481-500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Tui Na Acupressure Modulates Treg Immunosuppression via\n                    <scp>FoxP3<\/scp>\n                    \/\n                    <scp>mTORC1<\/scp>\n                    Signalling in\n                    <scp>ALS<\/scp>\n                    Mice"],"prefix":"10.1111","volume":"177","author":[{"given":"Jie","family":"Han","sequence":"first","affiliation":[{"name":"Rehabilitation Medicine Center\/Tuina Department Hubei Provincial Hospital of Traditional Chinese Medicine  Wuhan China"},{"name":"Affiliated Hospital of Hubei 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