{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T01:10:04Z","timestamp":1771031404202,"version":"3.50.1"},"reference-count":74,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:00:00Z","timestamp":1762560000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T00:00:00Z","timestamp":1762560000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tuberculosis (TB) remains a global health burden, particularly because of the limited efficacy of the Bacillus Calmette\u2010Gu\u00e9rin (BCG) vaccine against adult pulmonary TB. To improve immunogenicity, we developed a recombinant BCG strain expressing the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. tuberculosis<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010specific antigen Rv1507A (rBCG_Rv1507A) and evaluated its immune\u2010enhancing potential. rBCG_Rv1507A\u2010infected human PBMCs and murine macrophages exhibited enhanced co\u2010stimulatory marker expression and Th1\u2010skewed cytokine profiles in\u00a0vitro. The vaccine stimulated the expansion of T follicular helper (TFH) cells and both central and effector memory T cells. Intratracheal immunisation induced systemic and mucosal antibody responses, localized memory B cell formation, and enrichment of lung\u2010resident memory T cells in\u00a0vivo. Importantly, rBCG_Rv1507A promoted macrophage apoptosis and suppressed autophagy, which may support cross\u2010antigen presentation. Furthermore, it induces features of trained immunity, including hematopoietic progenitor expansion and metabolic reprogramming of macrophages. These immunological enhancements were compartmentalized to the lungs, the primary site of TB infection, due to mucosal delivery. Collectively, rBCG_Rv1507A demonstrated potential as a next\u2010generation TB vaccine by integrating durable adaptive memory with innate immune training. However, further studies are required to confirm its protective efficacy.\n                  <\/jats:p>","DOI":"10.1111\/imm.70063","type":"journal-article","created":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:00:47Z","timestamp":1762621247000},"page":"526-539","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Leveraging\n                    <scp>\n                      <i>Mycobacterium tuberculosis<\/i>\n                      Rv1507A\n                    <\/scp>\n                    Protein for Improved Immunogenicity in\n                    <scp>\n                      <i>Mycobacterium bovis<\/i>\n                      BCG\n                    <\/scp>\n                    Vaccine"],"prefix":"10.1111","volume":"177","author":[{"given":"Nilofer","family":"Naqvi","sequence":"first","affiliation":[{"name":"Cell Signaling and Inflammation Biology Lab, ICMR\u2010National Institute of Pathology  New Delhi India"},{"name":"Department of Life Science School of Basic Sciences and Research, Sharda University  Greater Noida India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwar","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Life Science School of Basic Sciences and Research, Sharda University  Greater Noida India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohd","family":"Shariq","sequence":"additional","affiliation":[{"name":"GITAM School of Science, GITAM University  Hyderabad India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yashika","family":"Ahuja","sequence":"additional","affiliation":[{"name":"Department of Life Science School of Basic Sciences and Research, Sharda University  Greater Noida India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dipendra K.","family":"Mitra","sequence":"additional","affiliation":[{"name":"Department of Transplant Immunology and Immunogenetics All India Institute of Medical Sciences  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2967-0791","authenticated-orcid":false,"given":"Seyed E.","family":"Hasnain","sequence":"additional","affiliation":[{"name":"Department of Life Science School of Basic Sciences and Research, Sharda University  Greater Noida India"},{"name":"Department of Biochemical Engineering and Biotechnology Indian Institute of Technology  New Delhi India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasreen Z.","family":"Ehtesham","sequence":"additional","affiliation":[{"name":"Cell Signaling and Inflammation Biology Lab, ICMR\u2010National Institute of Pathology  New Delhi India"},{"name":"Department of Life Science School of Basic Sciences and Research, Sharda University  Greater Noida India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409238.2024.2411264"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/adbi.202400174"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-024-05200-8"},{"key":"e_1_2_11_5_1","unstructured":"WHO \u201cGlobal Tuberculosis Report 2022 \u201d2022."},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00655-2019"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3704"},{"key":"e_1_2_11_8_1","unstructured":"WHO \u201cGlobal Tuberculosis Report 2024 \u201d."},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1183\/16000617.0264-2021"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lansea.2022.05.004"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00403-5"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-023-00750-7"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(19)30702-9"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.01.068"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12965"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00407"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60177-4"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI148546"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1100741"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01557-7"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2011.09.026"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107966"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.696491"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901367"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.11.6338-6343.2003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05249-11"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01199"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2024.2434026"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2025.112537"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02020-14"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.05.006"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1517822"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.564565"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.706081"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.594288"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06620-9"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13125"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.849583"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.1631"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI28139"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02571-09"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.636644"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.090446"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.4103\/aca.ACA_94_19"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines7040181"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0806510"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/32588"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.892701"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23126385"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132703"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101400"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1006-1029"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-032712-095939"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2321"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006080"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.2021495"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2024.2321494"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.126291"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01686-16"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12249"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.12703\/P6-13"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf1098"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2020.101978"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2017.97"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11050955"},{"key":"e_1_2_11_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1928"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.133"},{"key":"e_1_2_11_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm906"},{"key":"e_1_2_11_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01276"},{"key":"e_1_2_11_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-57090-4"},{"key":"e_1_2_11_71_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms9533"},{"key":"e_1_2_11_72_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1052374"},{"key":"e_1_2_11_73_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2404841121"},{"key":"e_1_2_11_74_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0285-6"},{"key":"e_1_2_11_75_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-025-01225-7"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.70063","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imm.70063","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.70063","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:28:27Z","timestamp":1771028907000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.70063"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,8]]},"references-count":74,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/imm.70063"],"URL":"https:\/\/doi.org\/10.1111\/imm.70063","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,8]]},"assertion":[{"value":"2025-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}