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The irradiation process triggers the recruitment and maladaptive activation of immune cells, thereby disrupting the delicate homeostasis of the jawbone. Despite its clinical significance, a comprehensive understanding of the osteoimmune microenvironment involved in underlying radiation\u2010induced jaw injury remains incompletely understood. In this study, we comprehensively profiled the transcriptional landscape of mandibular bone marrow at single\u2010cell resolution using single\u2010cell RNA sequencing (scRNA\u2010seq). Our analysis revealed a marked infiltration of conventional dendritic cells (cDCs). A specific subcluster of migratory dendritic cells (migDCs) characterised by the expression of genes related to maturation, migration and immune regulation was annotated. Following RT, these migDCs migrated to the draining lymph nodes. However, reduced secretion of neutrophil\u2010derived secreted phosphoprotein 1 (SPP1) was found to impair migDC development through the SPP1\/CD44\/NF\u2010\u03baB signalling pathway, leading to an immature cDC phenotype. We also observed weakened intercellular interactions between cDCs and T cells, contributing to an imbalanced and immunosuppressive osteoimmune microenvironment after radiation exposure. Overall, our study highlights the critical role of decreased SPP1 in modulating migDC function and its subsequent impact on jawbone immune dynamics following RT.<\/jats:p>","DOI":"10.1111\/imm.70088","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T11:06:08Z","timestamp":1766401568000},"page":"118-135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dysfunctional Dendritic Cells in Radiation\u2010Induced Jaw Injury: Insights From Single\u2010Cell Transcriptomic Analysis of the Osteoimmune Microenvironment"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8232-4812","authenticated-orcid":false,"given":"Mengting","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Orthodontics Laboratory of Dental Biomaterials and Tissue Regeneration, Shanghai Xuhui District Stomatological Hospital  Shanghai People's Republic of 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