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We investigated the metabolic changes in mouse bone marrow\u2010derived macrophages (BMDMs) upon Mtb infection and identified significant alterations in gene expression related to key metabolic pathways through RNA sequencing analyses. Among them, glycolysis\u2010related genes, including hypoxia\u2010inducible factor 1\u03b1 as a key regulator of glycolysis, are upregulated in Mtb\u2010infected BMDMs. To investigate whether glycolysis plays a critical role in reducing intracellular Mtb growth, we cultured Mtb\u2010infected BMDMs under high\u2010 or low\u2010glucose conditions. We found that high\u2010glucose conditions increased glycolytic enzyme levels, inducible nitric oxide synthase expression and proinflammatory cytokine production, reducing Mtb's intracellular survival. HIF1\u03b1 agonist treatment increased glycolysis, reactive oxygen species levels and proinflammatory cytokine production, enhancing bactericidal activity against Mtb. In contrast, inhibition of HIF1\u03b1 by a specific inhibitor FM19G11 leads to decreased glycolysis, reduced proinflammatory cytokine production and increased Mtb survival. Since succinate has been known to increase the stabilisation and activation of HIF1\u03b1, we added succinate to Mtb\u2010infected BMDMs to evaluate the function of succinate related to HIF1\u03b1 activation. As expected, succinate treatment enhanced glycolysis through HIF1\u03b1 stabilisation and shifted BMDMs to proinflammatory M1\u2010like phenotype. Our findings indicate that Mtb\u2010induced glycolysis plays a central role in the reduction of intracellular Mtb in BMDMs. Succinate is a key factor for HIF1\u03b1\u2010mediated glycolysis in Mtb\u2010infected BMDMs.\n                  <\/jats:p>","DOI":"10.1111\/imm.70116","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T07:29:26Z","timestamp":1770622166000},"page":"348-362","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>HIF1\u03b1<\/scp>\n                    Activates Glycolysis to Suppress\n                    <i>Mycobacterium tuberculosis<\/i>\n                    Growth in Mouse Bone Marrow\u2010Derived Macrophages"],"prefix":"10.1111","volume":"178","author":[{"given":"Junghwan","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"},{"name":"Translational Immunology Institute, Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaewhan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Ae","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"},{"name":"Translational Immunology Institute, Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tam Doan","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seoyeon","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Hwa","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Microbiology Chungnam National University  Daejeon South Korea"},{"name":"Department of Medical Science, College of Medicine Chungnam National University  Daejeon South Korea"},{"name":"Translational Immunology Institute, Chungnam National University  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