{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T17:22:52Z","timestamp":1781803372823,"version":"3.54.5"},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T00:00:00Z","timestamp":1770422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T00:00:00Z","timestamp":1770422400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100008347","name":"Bezmialem Vak\u0131f \u00dcniversitesi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Crimean\u2010Congo hemorrhagic fever virus (CCHFV) is a tick\u2010borne orthonairovirus associated with severe hemorrhagic fever and high mortality in humans. The genome is comprised of three segments of single\u2010stranded, negative\u2010sense RNA. The nucleocapsid (NP) protein, which is the focus of our study, encoded by the smallest (S) segment, plays a central role in viral RNA encapsidation and is known to be highly immunogenic, eliciting innate, humoral and cellular immune responses. This study aimed to identify the immunodominant regions of the NP that trigger T cell\u2010mediated immune responses. To this end, three truncated variants of the NP from the CCHFV Kelkit'06 strain [NPdelN(1\u2013135 aa), NPdelM(136\u2013295 aa) and NPdelC(296\u2013482 aa)] as well as the full\u2010length NP(1\u2013482 aa) were tested. Following mice immunisations, local lymph node cells were used as the source of lymphocytes and these cells were assessed to gauge the T cell responses. Stimulated splenocytes were analysed for lymphoproliferative responses and cytokine mRNA expression to evaluate T cell polarisation. Based on the data obtained, it appears that the amino\u2010terminal region (1\u2013135 amino acids) of NP is significantly more immunogenic than the other regions. In contrast, the carboxy\u2010terminal region (296\u2013482 aa) appears to play a suppressive role in cellular immune activation. Additionally, the middle region (136\u2013295 aa) of the NP is identified as being responsible for inducing Th17\u2010type cellular immune responses. In conclusion, this study points out to the specific regions of the CCHFV NP that are involved in shaping the cellular immune responses, representing a crucial step toward refining the structural elements contributing anti\u2010viral immunity and providing a sound ground for modulation efforts.<\/jats:p>","DOI":"10.1111\/imm.70119","type":"journal-article","created":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T10:10:41Z","timestamp":1770459041000},"page":"336-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Amino Terminal Region of Crimean\u2010Congo Hemorrhagic Fever Virus (\n                    <scp>CCHFV<\/scp>\n                    ) Nucleocapsid (\n                    <scp>NP<\/scp>\n                    ) Protein Contains Dominant Epitopes Recognised by Cellular Immunity"],"prefix":"10.1111","volume":"178","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1260-5736","authenticated-orcid":false,"given":"Aysegul","family":"Pirincal","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sercan","family":"Keskin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2066-0252","authenticated-orcid":false,"given":"Mehmet Ziya","family":"Doymaz","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Beykoz Institute of Life Sciences and Biotechnology, Bezmialem Vakif University  Istanbul T\u00fcrkiye"},{"name":"Department of Medical Microbiology, Medical School Bezmialem Vakif University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,7]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00871-9"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/v8040106"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16101547"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200808109"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184935"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA118.004976"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01627-12"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01555-12"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2022.105244"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13162708"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0009973"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-00815-6"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02156-20"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006149"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2015.1078045"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1233148"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.5.1587-1591.2002"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-010-3067-7"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.07.055"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","unstructured":"S.Keskin S.Pavel R.Sak et\u00a0al. \u201cComplete Protection Against Lethal Crimean\u2010Congo Hemorrhagic Fever Virus (CCHFV) Challenge in Mice Afforded by Immunization With Modified mRNA Encoding the Nucleocapsid Protein (NP) Alone \u201dpreprint bioRxiv 2025.","DOI":"10.1101\/2025.04.21.649815"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-025-01164-3"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2025.117145"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01558"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.165"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12121435"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.14235\/bas.galenos.2023.43925"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.07.022"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-024-00802-2"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit061"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612829666230904150808"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.2011.0016"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-024-01045-1"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02076-16"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiae395"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9020279"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204264"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.2679"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.190109"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30471"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201948157"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3665"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.03.003"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11142132"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.47665\/tb.37.4.1093"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/9140602"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24752"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/bbb\/zbad170"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.23697"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.60.2.708-717.1986"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2100939"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.21.11952-11961.2004"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.10.5216-5222.2004"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e20076"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.70119","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imm.70119","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.70119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T14:56:34Z","timestamp":1778252194000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.70119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,7]]},"references-count":53,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/imm.70119"],"URL":"https:\/\/doi.org\/10.1111\/imm.70119","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,7]]},"assertion":[{"value":"2025-12-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}