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Among the primary viral agents, Bovine herpesvirus 1 (BoHV\u20101) and Bovine respiratory syncytial virus (BRSV) play critical roles in initiating and exacerbating respiratory pathology. In this study, we engineered two recombinant Bovine herpesvirus 4 (BoHV\u20104)\u2010based vectors encoding chimeric antigens based on BoHV\u20101 glycoprotein D (gD) and BRSV fusion glycoprotein (gF), with the goal of developing a multivalent vaccine platform. Immunological evaluation in a murine model demonstrated that both vaccine constructs elicited robust humoral and cell\u2010mediated immune responses directed against both pathogens. Vaccination induced neutralising antibodies capable of inhibiting BoHV\u20101 and BRSV infection, as well as antigen\u2010specific T\u2010cell responses that mediated cytotoxic activity against target cells expressing either antigen. These findings provide proof\u2010of\u2010concept that chimeric antigens are effective in eliciting humoral and cellular immune response toward two main different pathogens, BoHV\u20101 and BRSV, and BoHV\u20104 is a versatile vector for the delivery of heterologous antigens. The demonstrated ability to induce both virus neutralisation and cytotoxic T\u2010cell activity supports the further development of BoHV\u20104\u2010vectored bivalent vaccines for BRD control, with potential application at improving livestock health and reducing reliance on antimicrobial treatments. Moreover, the present study highlights BoHV\u20104\u2010based vectors and chimeric peptides as a promising bivalent vaccine platform potentially translatable for controlling similar viruses like human respiratory syncytial virus (hRSV) and varicella zoster virus (VZV) across human populations.<\/jats:p>","DOI":"10.1111\/imm.70148","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:03:30Z","timestamp":1778857410000},"page":"54-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Inducing Antigen\u2010Specific and Functional Immune Responses in Mice Toward Bovine Herpesvirus 1 and Bovine Respiratory Syncytial Virus by Chimeric Peptides Delivered by Bovine Herpesvirus 4\u2010Based Vector"],"prefix":"10.1111","volume":"179","author":[{"given":"Antonino","family":"Di Lorenzo","sequence":"first","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences 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