{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:20:34Z","timestamp":1747185634439,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Paed Dentistry"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Caries is a worldwide distributed oral disease of multifactorial nature, with\u2009<jats:italic>Streptococcus mutans<\/jats:italic>\u2009being the most commonly isolated bacterial agent. The glycosyltransferases of this bacterium would play an essential role in the aetiology and pathogenesis of caries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>We explored how the glucosyltransferase\u2010B (gtf\u2010B) gene variability of <jats:italic>S.\u2009mutans<\/jats:italic> from children in central Argentina correlated with their caries experience and how these strains were genetically related to those of other countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Dental examinations were performed on 59 children; dmft and DMFT indexes were calculated. From stimulated saliva,\u2009<jats:italic>S. mutans<\/jats:italic>\u2009was grown and counted (CFU\/mL). From bacterial DNA, the gtf\u2010B gene was amplified and sequenced. Alleles were identified and their genealogical relationships established. Clinical, microbiological, and genetic variables were correlated with caries experience. Our sequences were included in a matrix with those from 16 countries (<jats:italic>n<\/jats:italic>\u2009=\u2009358); genealogical relationships among alleles were obtained. Population genetic analyses were performed for countries with &gt;20 sequences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean dmft\u2009+\u2009DMFT was 6.45. Twenty\u2010two gtf\u2010B alleles were identified here, which showed low genetic differentiation in the network. Caries experience was correlated with CFU\/mL, but not with allele variation. Low differentiation was found among the 70 alleles recovered from the 358 sequences and among the countries analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this study, caries experience in children was correlated with the number of CFU\/mL of\u2009<jats:italic>S. mutans<\/jats:italic>\u2009but not with the gtf\u2010B gene variability. Combined genetic analyses of worldwide strains support the theory that this bacterium experienced population expansions, probably associated with agriculture development and\/or food industrialization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ipd.13091","type":"journal-article","created":{"date-parts":[[2023,5,22]],"date-time":"2023-05-22T14:03:27Z","timestamp":1684764207000},"page":"615-624","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic variation in the <scp>glucosyltransferase\u2010B<\/scp> gene of <i>Streptococcus mutans<\/i> and its relationship with caries experience in children from C\u00f3rdoba, Argentina, and with strains from other countries"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0641-4725","authenticated-orcid":false,"given":"Fabiana Pia Marina","family":"Carletto\u2010K\u00f6rber","sequence":"first","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Soledad","family":"Acosta\u2010Jofr\u00e9","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas, F\u00edsicas y Naturales Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2842-461X","authenticated-orcid":false,"given":"Noelia Soledad","family":"Vera","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas, F\u00edsicas y Naturales Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0133-5094","authenticated-orcid":false,"given":"Mar\u00eda Rosa","family":"Mourelle\u2010Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Graciela","family":"Jim\u00e9nez","sequence":"additional","affiliation":[{"name":"Hospital Universitario de Maternidad y Neonatolog\u00eda, Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Enrique","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Hospital Universitario de Maternidad y Neonatolog\u00eda, Facultad de Ciencias M\u00e9dicas Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9116-0947","authenticated-orcid":false,"given":"Lila Susana","family":"Cornejo","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6761-3379","authenticated-orcid":false,"given":"Ra\u00fal Enrique","family":"Gonz\u00e1lez\u2010Ittig","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Exactas, F\u00edsicas y Naturales Universidad Nacional de C\u00f3rdoba  C\u00f3rdoba Argentina"},{"name":"Instituto de Diversidad y Ecolog\u00eda Animal (IDEA), CONICET\u2010UNC  C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000503309"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-021-00137-1"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.10.010"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000324598"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.47.59"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.54.1.519"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.04.007"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.9.3811-3817.1993"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009073"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2015.09.012"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056305"},{"volume-title":"Helsinki declaration of the world medical association. 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