{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:49:58Z","timestamp":1788871798479,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,3]],"date-time":"2024-01-03T00:00:00Z","timestamp":1704240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009592","name":"Beijing Municipal Science and Technology Commission","doi-asserted-by":"publisher","award":["Z181100001618008"],"award-info":[{"award-number":["Z181100001618008"]}],"id":[{"id":"10.13039\/501100009592","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021YFC2301000"],"award-info":[{"award-number":["2021YFC2301000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2021YFA0717000"],"award-info":[{"award-number":["2021YFA0717000"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Int J Paed Dentistry"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Children affected by severe early childhood caries (S\u2010ECC) usually need comprehensive caries treatment due to the extensive of caries. How the oral microbiome changes after caries therapy within the short\u2010term warrant further study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study aimed to investigate the short\u2010term impact of comprehensive caries treatment on the supragingival plaque microbiome of S\u2010ECC children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Design<\/jats:title><jats:p>Thirty\u2010three children aged 2\u20134\u2009years with severe caries (dt\u2009&gt;\u20097) were recruited. Comprehensive caries treatment was performed under general anesthesia in one session and included restoration, pulp treatment, extraction, and fluoride application. Supragingival plaque was sampled pre\u2010 and 1\u2010month posttreatment. The genomic DNA of the supragingival plaque was extracted, and bacterial 16S ribosomal RNA gene sequencing was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data showed that the microbial community evenness significantly decreased posttreatment. Furthermore, comprehensive caries treatment led to more diverse microbial structures among the subjects. The interbacterial interactions reflected by the microbial community's co\u2010occurrence network tended to be less complex posttreatment. Caries treatment increased the relative abundance of <jats:italic>Corynebacterium matruchotii<\/jats:italic>, <jats:italic>Corynebacterium durum<\/jats:italic>, <jats:italic>Actinomyces naeslundii<\/jats:italic>, and <jats:italic>Saccharibacteria HMT\u2010347<\/jats:italic>, as well as <jats:italic>Aggregatibacter HMT\u2010458<\/jats:italic> and <jats:italic>Haemophilus influenzae<\/jats:italic>. Meanwhile, the relative abundance of <jats:italic>Streptococcus mutans<\/jats:italic>, three species from Leptotrichia, <jats:italic>Neisseria bacilliformis<\/jats:italic>, and <jats:italic>Provotella pallens<\/jats:italic> significantly decreased posttreatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results suggested that comprehensive caries treatment may contribute to the reconstruction of a healthier supragingival microbiome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ipd.13151","type":"journal-article","created":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T05:56:03Z","timestamp":1704347763000},"page":"505-515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The short\u2010term impact of comprehensive caries treatment on the supragingival microbiome of severe early childhood caries"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2276-9286","authenticated-orcid":false,"given":"Jing","family":"Tian","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingqian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbin","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Resources, Institute of Microbiology Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenli","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6534-0976","authenticated-orcid":false,"given":"He","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsute","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology The Forsyth Institute  Cambridge Massachusetts USA"},{"name":"Department of Oral Medicine, Infection and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3333-9188","authenticated-orcid":false,"given":"Xuesong","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology The Forsyth Institute  Cambridge Massachusetts USA"},{"name":"Department of Oral Medicine, Infection and Immunity Harvard School of Dental Medicine  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-3319","authenticated-orcid":false,"given":"Man","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry Peking University School and Hospital of Stomatology, National Center for Stomatology, National Clinical Research Center for Oral Diseases, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374940080022001"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034515606045"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2016.1270614"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00450-19"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034518761644"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511421201"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.10.110"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0021-75572009000400005"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345211056247"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/baq013"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13040-015-0072-2"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02009"},{"key":"e_1_2_13_17_1","first-page":"61","article-title":"Plaque microbiome in caries\u2010active and caries\u2010free teeth by dentition","volume":"9","author":"Bhaumik D","year":"2022","journal-title":"JDR Clin Trans Res"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01145"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000517006"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-017-1056-1"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38346-3"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0200337"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1522149113"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2020.103539"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034517718819"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.940643"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100040501"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8111780"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1419038112"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215990119"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2114909119"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/20002297.2022.2046309"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.782825"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0139315"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/2774772"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph192215005"}],"container-title":["International Journal of Paediatric Dentistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ipd.13151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T07:07:47Z","timestamp":1723273667000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ipd.13151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,3]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1111\/ipd.13151"],"URL":"https:\/\/doi.org\/10.1111\/ipd.13151","archive":["Portico"],"relation":{},"ISSN":["0960-7439","1365-263X"],"issn-type":[{"value":"0960-7439","type":"print"},{"value":"1365-263X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,3]]},"assertion":[{"value":"2023-09-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}