{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T05:10:50Z","timestamp":1696309850706},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,20]],"date-time":"2013-03-20T00:00:00Z","timestamp":1363737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Characterization of the human respiratory syncytial virus (<jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content>) season at the local level has important implications for appropriate decisions on the time period for administration of specific prophylaxis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>(1) To describe five consecutive epidemic periods of <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> in an equatorial city of <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>razil and (2) to show preliminary data on genomic diversity of circulating <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>Nasopharyngeal aspirates of 2885 children attending the emergency room and wards of a public hospital were collected and screened by indirect immunofluorescence for <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> infections during five consecutive years (from <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>anuary 2004 to <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>ecember 2008). In addition, the genetic and antigenic variability of the <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> strains isolated was evaluated by partial nucleotide sequencing of the protein <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> was detected in 15\u00b78% of the analyzed samples. <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> seasons occurred in a restricted period of each year. The onset of each <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> season was variable (<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ebruary to <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ay), but the end always occurred in <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>uly. From the 456 <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> infections found, 86 cases with bronchiolitis were genotyped. Both <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> subgroups (<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>) cocirculated during the five epidemic periods. The 58 <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content>\u2010A strains grouped into two clades, <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>2 and <jats:styled-content style=\"fixed-case\">GA<\/jats:styled-content>5. In respect of the <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content>\u2010B strains, the 28 samples grouped into two clades: <jats:styled-content style=\"fixed-case\">GB<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">BA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> accounts for a substantial proportion of <jats:styled-content style=\"fixed-case\">ARI<\/jats:styled-content> in the study population. As in temperate countries, <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content> infections in this equatorial area of Brazil also cause seasonal yearly epidemics, and this has implications for prophylaxis strategies. The city of <jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>ortaleza follows the same worldwide trend of circulation of genotypes of <jats:styled-content style=\"fixed-case\">HRSV<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12104","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T04:14:00Z","timestamp":1363839240000},"page":"1128-1135","source":"Crossref","is-referenced-by-count":4,"title":["Respiratory syncytial virus epidemic periods in an equatorial city of <scp>B<\/scp>razil"],"prefix":"10.1111","volume":"7","author":[{"given":"Fernanda E. A.","family":"Moura","sequence":"first","affiliation":[{"name":"Federal University of Ceara Fortaleza Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne C. 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