{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T05:16:43Z","timestamp":1773033403263,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,23]],"date-time":"2015-04-23T00:00:00Z","timestamp":1429747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["200-2010-F-33132"],"award-info":[{"award-number":["200-2010-F-33132"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>We evaluated the feasibility of asking pregnant women to self\u2010collect and ship respiratory specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In a preliminary laboratory study, we compared the <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> cycle threshold (<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>) values of influenza A and B viruses incubated at 4 storage temperatures (from 4 to 35\u00b0C) for 6 time periods (8, 24, 48, 72, and 168\u00a0hours and 30\u00a0days), resulting in 24 conditions that were compared to an aliquot tested after standard freezing (\u221220\u00b0C) (baseline condition). In a subsequent pilot study, during January\u2013February, 2014, we delivered respiratory specimen collection kits to 53 pregnant women with a medically attended acute respiratory illness using three delivery methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> values were stable\u00a0after storage at temperatures &lt;27\u00b0C for up to 72\u00a0hours for influenza A viruses and 48\u00a0hours for influenza B viruses. Of 53 women who received kits during the pilot, 89% collected and shipped nasal swabs as requested. However, 30% (14\/47) of the women took over 2\u00a0days to collect and ship their specimen. The human control gene, ribonuclease P (<jats:styled-content style=\"fixed-case\">RN<\/jats:styled-content>ase P), was detected in 100% of nasal swab specimens. However, the mean <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> values for <jats:styled-content style=\"fixed-case\">RN<\/jats:styled-content>ase P (26\u00b75, 95% confidence interval [<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>]\u00a0=\u00a026\u00b70\u201327\u00b71) and for the 8 influenza A virus positives in our pilot (32\u00b72, 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\u00a0=\u00a028\u00b79\u201335\u00b75) were significantly higher than the <jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content>s observed in our 2010\u20132012 study using staff\u2010collected nasal pharyngeal swabs (<jats:italic>P<\/jats:italic>\u2010values\u00a0&lt;\u00a00\u00b701).<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Self\u2010collection of respiratory specimens is a promising research method, but further research is needed to quantify the sensitivity and specificity of the approach.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12309","type":"journal-article","created":{"date-parts":[[2015,2,25]],"date-time":"2015-02-25T07:09:12Z","timestamp":1424848152000},"page":"155-160","source":"Crossref","is-referenced-by-count":23,"title":["Results of a pilot study using self\u2010collected mid\u2010turbinate nasal swabs for detection of influenza virus infection among pregnant women"],"prefix":"10.1111","volume":"9","author":[{"given":"Mark G.","family":"Thompson","sequence":"first","affiliation":[{"name":"Influenza Division Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeannette R.","family":"Ferber","sequence":"additional","affiliation":[{"name":"Division of Research Kaiser Foundation Research Institute Oakland CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roxana","family":"Odouli","sequence":"additional","affiliation":[{"name":"Division of Research Kaiser Foundation Research Institute Oakland CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna","family":"David","sequence":"additional","affiliation":[{"name":"Marshfield Clinic Research Foundation Marshfield WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pat","family":"Shifflett","sequence":"additional","affiliation":[{"name":"Abt Associates, Inc. Cambridge MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer K.","family":"Meece","sequence":"additional","affiliation":[{"name":"Marshfield Clinic Research Foundation Marshfield WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison L.","family":"Naleway","sequence":"additional","affiliation":[{"name":"Center for Health Research Kaiser Permanente Northwest Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sam","family":"Bozeman","sequence":"additional","affiliation":[{"name":"Abt Associates, Inc. Cambridge MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah M.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Influenza Division Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicia M.","family":"Fry","sequence":"additional","affiliation":[{"name":"Influenza Division Centers for Disease Control and Prevention (CDC) Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De\u2010Kun","family":"Li","sequence":"additional","affiliation":[{"name":"Division of Research Kaiser Foundation Research Institute Oakland CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu327"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035166"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit750"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2011.04.009"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268807008412"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02235-09"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021335"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048508"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2011.07.005"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2014.08.004"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2051-2056.2002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2012.09.011"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2010.05.007"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1086\/675832"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01873-13"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/600383"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwm375"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/652241"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1092852900008981"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit736"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.11.4337-4339.2002"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03169.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu020"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"2324","DOI":"10.1093\/jac\/dkt203","article-title":"Virological self\u2010sampling to monitor influenza antiviral susceptibility in a community cohort","volume":"68","author":"Lackenby A","year":"2013","journal-title":"J Antimicrob Chemother"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2011.01.006"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Firv.12309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.12309","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T12:24:04Z","timestamp":1696249444000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.12309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,23]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1111\/irv.12309"],"URL":"https:\/\/doi.org\/10.1111\/irv.12309","archive":["Portico"],"relation":{},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,23]]}}}