{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:17:39Z","timestamp":1787854659820,"version":"build-2784847793"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T00:00:00Z","timestamp":1491350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008482","name":"Association of Public Health Laboratories","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008482","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["1\u2010U01\u2010CI000298"],"award-info":[{"award-number":["1\u2010U01\u2010CI000298"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Detections of influenza A subtype\u2010specific antibody responses are often complicated by the presence of cross\u2010reactive antibodies. We developed two novel multiplex platforms for antibody detection. The multiplexed magnetic fluorescence microsphere immunoassay (MAGPIX) is a high\u2010throughput laboratory\u2010based assay. Chembio Dual Path Platform (DPP) is a portable and rapid test that could be used in the field.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twelve recombinant globular head domain hemagglutinin (GH HA1) antigens from A(H1N1)pdm09 (pH1N1), A(H2N2), A(H3N2), A(H5N1), A(H7N9), A(H9N2), A(H13N9), B\/Victoria lineage, B\/Yamagata lineage viruses, and protein A control were used. Human sera from U.S. residents either vaccinated (with H5N1 or pH1N1) or infected with pH1N1 influenza viruses and sera from live bird market workers in Bangladesh (BDPW) were evaluated. GH HA1 antigens and serum adsorption using full ectodomain recombinant hemagglutinins from A(pH1N1) and A(H3N2) were introduced into the platforms to reduce cross\u2010reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum adsorption reduced cross\u2010reactivity to novel subtype HAs. Compared to traditional hemagglutination inhibition or microneutralization assays, when serum adsorption and the highest fold rise in signals were used to determine positivity, the correct subtype\u2010specific responses were identified in 86%\u2010100% of U.S. residents exposed to influenza antigens through vaccination or infection (N=49). For detection of H5N1\u2010specific antibodies in sera collected from BDPW, H5 sensitivity was 100% (six of six) for MAGPIX, 83% (five of six) for DPP, H5 specificity was 100% (15\/15), and cross\u2010reactivity against other subtype was 0% (zero of six) for both platforms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>MAGPIX and DPP platforms can be utilized for high\u2010throughput and in\u2010field detection of novel influenza virus infections.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12449","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T21:10:30Z","timestamp":1487279430000},"page":"289-297","source":"Crossref","is-referenced-by-count":19,"title":["Novel multiplex assay platforms to detect influenza A hemagglutinin subtype\u2010specific antibody responses for high\u2010throughput and in\u2010field applications"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4556-8551","authenticated-orcid":false,"given":"Zhu\u2010Nan","family":"Li","sequence":"first","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica F.","family":"Trost","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"},{"name":"Department of Microbiology and Immunology Emory University  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly M.","family":"Weber","sequence":"additional","affiliation":[{"name":"Battelle Memorial Institute  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth H.","family":"LeMasters","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharifa","family":"Nasreen","sequence":"additional","affiliation":[{"name":"Centre for Communicable Diseases The International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javan","family":"Esfandiari","sequence":"additional","affiliation":[{"name":"Chembio Diagnostic Systems, Inc  Medford NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo H.","family":"Gunasekera","sequence":"additional","affiliation":[{"name":"Chembio Diagnostic Systems, Inc  Medford NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"McCausland","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Emory University  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharine","family":"Sturm\u2010Ramirez","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"},{"name":"Centre for Communicable Diseases The International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Wrammert","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology Emory University  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Gregory","sequence":"additional","affiliation":[{"name":"Battelle Memorial Institute  Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vic","family":"Veguilla","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Stevens","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph D.","family":"Miller","sequence":"additional","affiliation":[{"name":"Influenza Division National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention  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