{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T16:44:00Z","timestamp":1765039440445,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The large consumption of neuraminidase inhibitors (<jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content>s) for the treatment of influenza virus infections places Japan at risk of becoming the epicenter of the global spread of <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content>\u2010resistant viruses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To clarify <jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content> amino acid mutations of epidemic influenza viruses in Japan and their related <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content> resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 1791 samples, including 396 A\/H1N1pdm09, 1117 A\/H3N2, and 278 B isolates, were collected to determine of their 50% inhibitory concentration (<jats:styled-content style=\"fixed-case\">IC<\/jats:styled-content><jats:sub>50<\/jats:sub>) values by <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content>s (oseltamivir, zanamivir, peramivir, and laninamivir) during the Japanese seasons from 2011\u20102012 to 2016\u20102017. Then, 380 samples including 49 A\/H1N1pdm09, 251 A\/H3N2, and 80 B isolates were sequenced for the entire <jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Neuraminidase inhibitor\u2010resistant A\/H1N1pdm09 viruses were detected at a frequency of 1.3% (5\/396 isolates) in the epidemic seasons. None of the A\/H3N2 and B viruses developed resistance to any of the four <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content>s during the six seasons. Only five and 13 <jats:styled-content style=\"fixed-case\">AA<\/jats:styled-content> mutations were detected in the <jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content> catalytic sites of A\/H1N1pdm09 and A\/H3N2 viruses, respectively. No mutations were observed in the catalytic sites of B viruses. Four of the five mutations in the catalytic sites of A\/H1N1pdm09 consisted of H275Y, which was related to high resistance to oseltamivir and peramivir. Most (10\/13) of the catalytic site mutations in A\/H3N2 were associated with <jats:styled-content style=\"fixed-case\">MDCK<\/jats:styled-content>\u2010passaged induction (D151G\/N). Finally, no mutations related to substantial <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content> resistance were detected in the A\/H3N2 and B viruses examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that the <jats:styled-content style=\"fixed-case\">NA<\/jats:styled-content> catalytic sites of influenza viruses are well preserved. Even in Japan, no spread of <jats:styled-content style=\"fixed-case\">NAI<\/jats:styled-content>\u2010resistant viruses has been observed, and A\/H1N1pdm09 viruses carrying H275Y remain limited.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12624","type":"journal-article","created":{"date-parts":[[2018,12,12]],"date-time":"2018-12-12T06:39:09Z","timestamp":1544596749000},"page":"115-122","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Consecutive influenza surveillance of neuraminidase mutations and neuraminidase inhibitor resistance in Japan"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8804-3181","authenticated-orcid":false,"given":"Yong","family":"Chong","sequence":"first","affiliation":[{"name":"Medicine and Biosystemic Science Kyushu University Graduate School of Medical Sciences  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine Kyushu University Hospital  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongchon","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Laboratory Medicine Kyushu University Hospital  Fukuoka Japan"},{"name":"Department of Clinical Chemistry and Laboratory Medicine Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideyuki","family":"Ikematsu","sequence":"additional","affiliation":[{"name":"Japan Physicians Association Influenza Study Group  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)11433-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S105473"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/648424"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2009.07.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0910060"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2017.08.004"},{"key":"e_1_2_7_8_1","unstructured":"National Institute of Infectious Diseases.https:\/\/www.niid.go.jp\/niid\/en\/influ-resist-e\/8092-flu-r-e20180607.html. Accessed June 21 2018."},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2013.07.002"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.05.004"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2014.08.030"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2016.06.002"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2018.04.009"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.2990-2995.1998"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2015.02.003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00333-08"},{"key":"e_1_2_7_17_1","first-page":"369e374","article-title":"Meetings of the WHO working group on surveillance of influenza antiviral susceptibility \u2010 Geneva, November 2011 and June 2012","volume":"87","author":"WHO","year":"2012","journal-title":"Wkly Epidemiol Rec"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01109-09"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03265-13"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.11.084"},{"key":"e_1_2_7_21_1","unstructured":"WHO.2014.http:\/\/www.who.int\/influenza\/gisrs_laboratory\/antiviral_susceptibility\/NAI_Reduced_Susceptibility_Marker_Table_WHO.pdf. Accessed June 21 2018."},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00458-10"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079252"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01364-13"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12113"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00477-06"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.06.001"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis337"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.1.20666"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004065"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04836-14"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06956"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Firv.12624","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12624","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.12624","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12624","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T12:43:14Z","timestamp":1694695394000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.12624"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,9]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1111\/irv.12624"],"URL":"https:\/\/doi.org\/10.1111\/irv.12624","archive":["Portico"],"relation":{},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"type":"print","value":"1750-2640"},{"type":"electronic","value":"1750-2659"}],"subject":[],"published":{"date-parts":[[2019,1,9]]},"assertion":[{"value":"2018-07-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}