{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T01:53:00Z","timestamp":1768096380295,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T00:00:00Z","timestamp":1567641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000200","name":"United States Agency for International Development","doi-asserted-by":"publisher","award":["EPT-2"],"award-info":[{"award-number":["EPT-2"]}],"id":[{"id":"10.13039\/100000200","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["HHSN272201400006C"],"award-info":[{"award-number":["HHSN272201400006C"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004791","name":"Shenzhen Graduate School, Peking University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004791","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004420","name":"United Nations","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004420","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Avian influenza A (H7N9) remains circulating in China. For countries at risk of introduction of H7N9, such as Vietnam, early detection of H7N9 virus is essential for the early containment of the virus. Insulated isothermal reverse transcriptase PCR (iiRT\u2010PCR) is a portable PCR system that can be deployed under field conditions to identify pathogens at the sampling site. Applying PCR at the sampling site will greatly reduce the time to obtain a diagnostic result which allows the veterinary authority to take immediate action to contain disease spreading.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine analytical and diagnostic sensitivity and specificity of the portable iiRT\u2010PCR for H7N9 virus detection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A panel of 59 virus isolates, including H7N9, avian influenza viruses of subtype H1 to H13, swine and human influenza viruses, Newcastle disease virus, and infectious bursal disease virus, were tested by H7 and N9 iiRT\u2010PCR reagents, using probes and primers specific to H7 or N9, in comparison with laboratory\u2010based real\u2010time RT\u2010PCR assays to determine analytical sensitivity and specificity. Fifty oropharyngeal samples from experimentally infected chicken and ducks with H7N9 and 50 non\u2010infected control swabs were tested by the H7 iiRT\u2010PCR to determine diagnostic sensitivity and specificity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The H7 and N9 iiRT\u2010PCR reagents yielded comparable levels of analytical sensitivity and specificity with real\u2010time RT\u2010PCR for the detection of H7N9 virus. Diagnostic sensitivity and specificity of H7 iiRT\u2010PCR were 98% and 100%, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The observed high sensitivity and specificity of iiRT\u2010PCR for H7N9 detection show its potential for early detection of H7N9 in risk\u2010based surveillance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12646","type":"journal-article","created":{"date-parts":[[2019,9,5]],"date-time":"2019-09-05T13:11:29Z","timestamp":1567689089000},"page":"610-617","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A field\u2010deployable insulated isothermal RT\u2010PCR assay for identification of influenza A (H7N9) shows good performance in the laboratory"],"prefix":"10.1111","volume":"13","author":[{"given":"Ken","family":"Inui","sequence":"first","affiliation":[{"name":"Food and Agriculture Organization of the United Nations (FAO) Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tung","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Animal Health Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsin\u2010Jou","family":"Tseng","sequence":"additional","affiliation":[{"name":"GeneReach USA Lexington Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ChuanFu Mark","family":"Tsai","sequence":"additional","affiliation":[{"name":"GeneReach USA Lexington Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Long","family":"Tsai","sequence":"additional","affiliation":[{"name":"GeneReach USA Lexington Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Chung","sequence":"additional","affiliation":[{"name":"GeneReach USA Lexington Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawin","family":"Padungtod","sequence":"additional","affiliation":[{"name":"Food and Agriculture Organization of the United Nations (FAO) Hanoi Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huachen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Joint Institute of Virology (Shantou University \u2013 The University of Hong Kong) Shantou China"},{"name":"State Key Laboratory of Emerging Infectious Diseases, School of Public Health The University of Hong Kong Hong Kong China"},{"name":"Shenzhen Third People\u2019s Hospital Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Guan","sequence":"additional","affiliation":[{"name":"Joint Institute of Virology (Shantou University \u2013 The University of Hong Kong) Shantou China"},{"name":"State Key Laboratory of Emerging Infectious Diseases, School of Public Health The University of Hong Kong Hong Kong China"},{"name":"Shenzhen Third People\u2019s Hospital Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wantanee","family":"Kalpravidh","sequence":"additional","affiliation":[{"name":"Food and Agriculture Organization of the United Nations (FAO) Regional office for Asia and the Pacific Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5705-7572","authenticated-orcid":false,"given":"Filip","family":"Claes","sequence":"additional","affiliation":[{"name":"Food and Agriculture Organization of the United Nations (FAO) Regional office for Asia and the Pacific Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,5]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"WHO 2018.http:\/\/iris.wpro.who.int\/bitstream\/handle\/10665.1\/14179\/AI-20180126.pdf. 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