{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T19:10:18Z","timestamp":1790795418451,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T00:00:00Z","timestamp":1569024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Bioaerosol sampling devices are necessary for the characterization of infectious bioaerosols emitted by naturally\u2010infected hosts with acute respiratory virus infections. Assessment of these devices under multiple experimental conditions will provide insight for device use.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The primary objective of this study was to assess and compare bioaerosol sampling devices using a) an in vitro, environmentally\u2010controlled artificial bioaerosol system at a range of different RH conditions and b) an in vivo bioaerosol system of influenza virus\u2010infected ferrets under controlled environmental conditions. Secondarily, we also sought to examine the impact of NSAIDs on bioaerosol emission in influenza virus\u2010infected ferrets to address its potential as a determinant of bioaerosol emission.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the performance of low and moderate volume bioaerosol samplers for the collection of viral RNA and infectious influenza virus in vitroand in vivo using artificial bioaerosols and the ferret model of influenza virus infection. The following samplers were tested: the polytetrafluoroethylene filter (PTFE filter), the 2\u2010stage National Institute of Occupational Safety and Health cyclone sampler (NIOSH cyclone sampler) and the 6\u2010stage viable Andersen impactor (Andersen impactor).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PTFE filter and NIOSH cyclone sampler collected similar amounts of viral RNA and infectious virus from artificially\u2010generated aerosols under a range of relative humidities (RH). Using the ferret model, the PTFE filter, NIOSH cyclone sampler and the Andersen impactor collected up to 3.66 log<jats:sub>10<\/jats:sub>copies of RNA\/L air, 3.84 log<jats:sub>10<\/jats:sub>copies of RNA\/L air and 6.09 log<jats:sub>10<\/jats:sub>copies of RNA\/L air respectively at peak recovery. Infectious virus was recovered from the PTFE filter and NIOSH cyclone samplers on the peak day of viral RNA recovery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The PTFE filter and NIOSH cyclone sampler are useful for influenza virus RNA and infectious virus collection and may be considered for clinical and environmental settings.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12678","type":"journal-article","created":{"date-parts":[[2019,9,21]],"date-time":"2019-09-21T08:16:46Z","timestamp":1569053806000},"page":"564-573","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Evaluation of bioaerosol samplers for the detection and quantification of influenza virus from artificial aerosols and influenza virus\u2013infected ferrets"],"prefix":"10.1111","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2891-6670","authenticated-orcid":false,"given":"Christian","family":"Bekking","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Faculty of Medicine University of Toronto Toronto Ontario Canada"},{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"Yip","sequence":"additional","affiliation":[{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Groulx","sequence":"additional","affiliation":[{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Doggett","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Faculty of Medicine University of Toronto Toronto Ontario Canada"},{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mairead","family":"Finn","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Faculty of Medicine University of Toronto Toronto Ontario Canada"},{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5012-2311","authenticated-orcid":false,"given":"Samira","family":"Mubareka","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathobiology Faculty of Medicine University of Toronto Toronto Ontario Canada"},{"name":"Biological Sciences Sunnybrook Research Institute Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,9,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1213362"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuv039"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100768108"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/378292"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/15459620590918466"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.4161\/viru.27172"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.54.030192.001555"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2012.07.005"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/00016480252814207"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03544-13"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/188430a0"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057485"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/145.4.520"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.2570"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2016.03.017"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1em10607d"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0668.2009.00609.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofw259"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033974"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2017.06.020"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis773"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148669"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ321"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072226"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638717700221"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw180"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/annhyg\/mel073"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002691"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/02786820802621232"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis237"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149083"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0135675"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.76.5.471-484.1958"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2007.00020.x"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.02.041"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1086\/322801"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1177238"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1177127"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.007823"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00022-16"},{"key":"e_1_2_6_42_1","unstructured":"World Health Organization.CDC protocol of realtime RTPCR for influenza A (H1N1). 2009;http:\/\/www.who.int\/csr\/resources\/publications\/swineflu\/CDCRealtimeRTPCR_SwineH1Assay-2009_20090430.pdf?ua=1"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.9.4420-4429.2002"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3nr01525d"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.11.002"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw239"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1086\/429634"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2016.21.35.30331"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.buildenv.2017.10.037"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046789"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00551-18"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00325-08"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06645-11"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01317930"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1086\/597073"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604157103"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00552-19"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.5326\/0410298"},{"key":"e_1_2_6_59_1","first-page":"444","article-title":"The relation of pyrexia and nasal inflammatory response to virus levels in nasal washings of ferrets infected with influenza viruses of differing virulence","volume":"58","author":"Toms GL","year":"1977","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-009-0016-x"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy221"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12678","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.12678","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.12678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T02:23:25Z","timestamp":1694226205000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.12678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,9,21]]},"references-count":60,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/irv.12678"],"URL":"https:\/\/doi.org\/10.1111\/irv.12678","archive":["Portico"],"relation":{},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,9,21]]},"assertion":[{"value":"2019-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-09-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}