{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:19:27Z","timestamp":1747185567538,"version":"3.40.5"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,13]],"date-time":"2019-10-13T00:00:00Z","timestamp":1570924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000016","name":"U.S. Department of Health and Human Services","doi-asserted-by":"publisher","award":["HHSO100201400010C","HHSO100201500024C"],"award-info":[{"award-number":["HHSO100201400010C","HHSO100201500024C"]}],"id":[{"id":"10.13039\/100000016","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip\u20108G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip\u20108G Insight uses whole gene segment RT\u2010PCR\u2010based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network\u2010based data interpretation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective of this study was to verify and validate the performance of the FluChip\u20108G Insight assay for the detection and positive identification of human and animal origin non\u2010seasonal influenza A specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We evaluated the ability of the FluChip\u20108G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non\u2010seasonal influenza A strains (49 unique subtypes).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FluChip\u20108G Insight demonstrated a positive percent agreement \u226593% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The FluChip\u20108G Insight neural network\u2010based algorithm used for virus identification performed well over a data set of 297 na\u00efve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.12683","type":"journal-article","created":{"date-parts":[[2019,10,14]],"date-time":"2019-10-14T04:53:58Z","timestamp":1571028838000},"page":"55-60","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["FluChip\u20108G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay"],"prefix":"10.1111","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9801-101X","authenticated-orcid":false,"given":"Evan","family":"Toth","sequence":"first","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica D.","family":"Dawson","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amber W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert S.","family":"Stoughton","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca H.","family":"Blair","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"James E.","family":"Johnson","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Slinskey","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Fessler","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine B.","family":"Smith","sequence":"additional","affiliation":[{"name":"Influenza Division the Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Talbot","sequence":"additional","affiliation":[{"name":"Influenza Division the Centers for Disease Control and Prevention Atlanta GA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kathy","family":"Rowlen","sequence":"additional","affiliation":[{"name":"InDevR, Inc. Boulder CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,13]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"cdc.gov[internet].Centers for disease control and prevention; [cited 2019 Apr 3].https:\/\/www.cdc.gov\/flu\/about\/burden\/2017-2018.htm"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2407.180028"},{"issue":"12","key":"e_1_2_7_4_1","first-page":"2596","article-title":"Readiness for responding to a severe pandemic 100 years after 1918","volume":"187","author":"Jester B","year":"2018","journal-title":"Amer. J. 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