{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:57:03Z","timestamp":1787061423591,"version":"build-2736575974"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T00:00:00Z","timestamp":1571961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fk0108124"],"award-info":[{"award-number":["JP18fk0108124"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP18fk0108212"],"award-info":[{"award-number":["JP18fk0108212"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007077","name":"National Institute of Biomedical Innovation","doi-asserted-by":"publisher","award":["08\u201016"],"award-info":[{"award-number":["08\u201016"]}],"id":[{"id":"10.13039\/501100007077","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["h23\u2010shinkou\u2010shitei\u2010021"],"award-info":[{"award-number":["h23\u2010shinkou\u2010shitei\u2010021"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cell\u2010based influenza vaccines can solve the problem of the frequent occurrence of egg adaptation\u2013associated antigenic changes observed in egg\u2010based vaccines. Seed viruses for cell\u2010based vaccines can be prepared from clinical specimens by cell culture; however, clinical samples risk harboring respiratory viruses other than influenza virus. Therefore, it is necessary to investigate the patterns of co\u2010infection in clinical samples and explore whether cell culture technology can selectively propagate influenza viruses from samples containing other respiratory viruses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 341 clinical specimens were collected from patients with influenza or influenza\u2010like illness and analyzed by ResPlex II assay to detect 18 respiratory viruses. The patterns of co\u2010infection were statistically analyzed with Fisher's exact test. The samples with double or triple infections were passaged in suspension MDCK cells (MDCK\u2010S), adherent MDCK cells (MDCK\u2010A), and LLC\u2010MK2D cells. Cell\u2010passaged samples were analyzed by ResPlex II assay again to investigate whether each cell line could amplify influenza viruses and eliminate other respiratory viruses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Double infections were detected in 8.5% and triple infections in 0.9% of the collected clinical specimens. We identified four pairs of viruses with significant correlation. For all samples with double and triple infection, MDCK\u2010S and MDCK\u2010A could selectively propagate influenza viruses, while eliminating all contaminating viruses. In contrast, LLC\u2010MK2D showed lower isolation efficiency for influenza virus and higher isolation efficiency for coxsackievirus\/echovirus than MDCK\u2010S and MDCK\u2010A.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Both MDCK\u2010S and MDCK\u2010A are considered suitable for the preparation of influenza vaccine seed viruses without adventitious agents or egg\u2010adaptation mutations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.12694","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T05:41:09Z","timestamp":1571982069000},"page":"204-209","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparison of suspension MDCK cells, adherent MDCK cells, and LLC\u2010MK2 cells for selective isolation of influenza viruses to be used as vaccine seeds"],"prefix":"10.1111","volume":"14","author":[{"given":"Yuichi","family":"Harada","sequence":"first","affiliation":[{"name":"Influenza Virus Research Center National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Influenza Virus Research Center National Institute of Infectious Diseases Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Trusheim","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GmbH Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Roth","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics GmbH Marburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsumi","family":"Mizuta","sequence":"additional","affiliation":[{"name":"Yamagata Prefectural 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