{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:56:13Z","timestamp":1789692973058,"version":"4.0.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T00:00:00Z","timestamp":1597881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1111925"],"award-info":[{"award-number":["APP1111925"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Molecular assays based on reverse transcription\u2010polymerase chain reaction (RT\u2010PCR) provide reliable results for the detection of respiratory pathogens, although diagnostic agreement varies. This study determined the agreement between the RT\u2010PCR assays (Xpert\n                      <jats:sup>\u00ae<\/jats:sup>\n                      Flu\/RSV vs Allplex\n                      <jats:sup>\u2122<\/jats:sup>\n                      RP1) in detecting influenza A, influenza B, and respiratory syncytial viruses (RSVs) in clinical practice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We retrospectively identified 914 patient encounters where testing with both Xpert\n                      <jats:sup>\u00ae<\/jats:sup>\n                      Flu\/RSV and Allplex\n                      <jats:sup>\u2122<\/jats:sup>\n                      RP1 was undertaken between October 2015 and September 2019 in seven hospitals across New South Wales, Australia. The diagnostic agreement of the two assays was evaluated using positive percent agreement, negative percent agreement, and prevalence and bias\u2010adjusted kappa.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The positive percent agreement was 95.1% for influenza A, 87.5% for influenza B, and 77.8% for RSV. The negative percent agreement was 99.4% for influenza A, 99.9% for influenza B, and 100% for RSV. The prevalence and bias\u2010adjusted kappa was 0.98 for influenza A, 0.99 for influenza B, and 0.97 for RSV. In a sensitivity analysis, the positive percent agreement values were significantly higher during the non\u2010influenza season than the influenza season for influenza B and RSV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The Xpert\n                      <jats:sup>\u00ae<\/jats:sup>\n                      Flu\/RSV and Allplex\n                      <jats:sup>\u2122<\/jats:sup>\n                      RP1 demonstrated a high diagnostic agreement for all three viruses assessed. The seasonal variation in the positive percent agreement of the two assays for influenza B and RSV may have been due to lower numbers assessed, variability in the virology of infections outside the peak season, or changes in the physiology of the infected host in different seasons.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.12799","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T16:12:48Z","timestamp":1598544768000},"page":"245-253","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Cepheid Xpert\n                    <sup>\u00ae<\/sup>\n                    Flu\/RSV and Seegene Allplex\n                    <sup>\u2122<\/sup>\n                    RP1 show high diagnostic agreement for the detection of influenza A\/B and respiratory syncytial viruses in clinical practice"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9740-6319","authenticated-orcid":false,"given":"Nasir","family":"Wabe","sequence":"first","affiliation":[{"name":"Centre for Health Systems and Safety Research Australian Institute of Health Innovation Macquarie University  North Ryde NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Lindeman","sequence":"additional","affiliation":[{"name":"NSW Health Pathology  Chatswood NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey J.","family":"Post","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Prince of Wales Hospital and Community Health Services  Randwick NSW Australia"},{"name":"Prince of Wales Clinical School University of New South Wales  Kensington NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Rawlinson","sequence":"additional","affiliation":[{"name":"NSW Health Pathology Randwick Prince of Wales Hospital and Community Health Services  Randwick NSW Australia"},{"name":"School of Medical Sciences School of Biotechnology and Biomolecular Sciences University of New South Wales  Kensington NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Miao","sequence":"additional","affiliation":[{"name":"Centre for Health Systems and Safety Research Australian Institute of Health Innovation Macquarie University  North Ryde NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanna I.","family":"Westbrook","sequence":"additional","affiliation":[{"name":"Centre for Health Systems and Safety Research Australian Institute of Health Innovation Macquarie University  North Ryde NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Georgiou","sequence":"additional","affiliation":[{"name":"Centre for Health Systems and Safety Research Australian Institute of Health Innovation Macquarie University  North Ryde NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268813002471"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268818000286"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30938-8"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(18)30496-X"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.09.020421"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)33293-2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02045-16"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01727-18"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/lmx038"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5694\/mja2.50049"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2017.08.001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2010.11.019"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PEC.0000000000000721"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00556-09"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2018.05.006"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499313"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2018.38.1.46"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00972-15"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.01.008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00084-16"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2019-030104"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2012.031"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0895-4356(93)90018-V"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/sim.7273"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2017.00307"},{"key":"e_1_2_9_27_1","unstructured":"Influenza 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