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The protocol can be used in laboratories with basic Sanger sequencing capabilities and allows rapid \u201cat source\u201d screening for SARS\u2010CoV\u20102 variants, notably those of concern. The protocol has been applied for surveillance, with clinical specimens collected in either nucleic acid preservation lysis\u2010mix or virus transport medium, and research involving cultured viruses, and can yield data of public health importance in a timely manner.<\/jats:p>","DOI":"10.1111\/irv.12892","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T02:40:32Z","timestamp":1628044832000},"page":"707-710","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A Sanger sequencing protocol for SARS\u2010CoV\u20102 S\u2010gene"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2818-5089","authenticated-orcid":false,"given":"Rodney S.","family":"Daniels","sequence":"first","affiliation":[{"name":"Worldwide Influenza Centre (WIC: a WHO Collaborating Centre for Reference and Research on Influenza) The Francis Crick Institute  London 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