{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T17:08:36Z","timestamp":1785863316891,"version":"3.56.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T00:00:00Z","timestamp":1642377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004348","name":"Stockholms L\u00e4ns Landsting","doi-asserted-by":"publisher","award":["SLL ALF20190536"],"award-info":[{"award-number":["SLL ALF20190536"]}],"id":[{"id":"10.13039\/501100004348","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The knowledge on the concentration of viral particles in exhaled breath is limited. The aim of this study was to explore if severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) can be detected in aerosol from subjects with the coronavirus disease 2019 (COVID\u201019) during various types of breathing and coughing and how infection with SARS\u2010CoV\u20102 may influence the number and size of exhaled aerosol particles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We counted and collected endogenous particles in exhaled breath in subjects with COVID\u201019 disease by two different impaction\u2010based methods, during 20 normal breaths, 10 airway opening breaths, and three coughs, respectively. Breath samples were analyzed with reverse transcription real\u2010time polymerase chain reaction (RT\u2010PCR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Detection of RNA in aerosol was possible in 10 out of 25 subjects. Presence of virus RNA in aerosol was mainly found in cough samples (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098), but also in airway opening breaths (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20093) and in normal breaths (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20094), with no overlap between the methods. No association between viral load in aerosol and number exhaled particles &lt;5\u00a0\u03bcm was found. Subjects with COVID\u201019 exhaled less particles than healthy controls during normal breathing and airway opening breaths (all\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05), but not during cough.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>SARS\u2010CoV\u20102 RNA can be detected in exhaled aerosol, sampled during a limited number of breathing and coughing procedures. Detection in aerosol seemed independent of viral load in the upper airway swab as well as of the exhaled number of particles. The infectious potential of the amount of virus detected in aerosol needs to be further explored.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.12964","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T04:31:00Z","timestamp":1642393860000},"page":"402-410","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Severe acute respiratory syndrome coronavirus 2 can be detected in exhaled aerosol sampled during a few minutes of breathing or coughing"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4453-4680","authenticated-orcid":false,"given":"Emilia","family":"Viklund","sequence":"first","affiliation":[{"name":"Occupational and Environmental Medicine, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Spela","family":"Kokelj","sequence":"additional","affiliation":[{"name":"Occupational and Environmental Medicine, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Larsson","sequence":"additional","affiliation":[{"name":"Occupational and Environmental Medicine, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rickard","family":"Nord\u00e9n","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Clinical Microbiology Sahlgrenska University Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Andersson","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olof","family":"Beck","sequence":"additional","affiliation":[{"name":"Department of Clinical Neuroscience Karolinska Institute  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Westin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Clinical Microbiology Sahlgrenska University Hospital  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna\u2010Carin","family":"Olin","sequence":"additional","affiliation":[{"name":"Occupational and Environmental Medicine, School of Public Health and Community Medicine, Institute of Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ina.12751"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.buildenv.2021.107788"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/148.3.778"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaerosci.2008.11.002"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00873.2009"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.resp.2012.10.010"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/jamp.2013.1104"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0227980"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd9149"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"ColemanKK TayDJW Sen TanK et al.Viral load of SARS\u2010CoV\u20102 in respiratory aerosols emitted by COVID\u201019 patients while breathing talking and singing. 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