{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:55:09Z","timestamp":1789692909379,"version":"4.0.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1127419"],"award-info":[{"award-number":["OPP1127419"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>External quality assessments (EQAs) for the molecular detection of human respiratory syncytial virus (RSV) are necessary to ensure the standardisation of reliable results. The Phase II, 2019\u20132020 World Health Organization (WHO) RSV EQA included 28 laboratories in 26 countries. The EQA panel evaluated performance in the molecular detection and subtyping of RSV\u2010A and RSV\u2010B. This manuscript describes the preparation, distribution, and analysis of the 2019\u20132020 WHO RSV EQA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Panel isolates underwent whole genome sequencing and in silico primer matching. The final panel included nine contemporary, one historical virus and two negative controls. The EQA panel was manufactured and distributed by the UK National External Quality Assessment Service (UK NEQAS). National laboratories used WHO reference assays developed by the United States Centers for Disease Control and Prevention, an RSV subtyping assay developed by the Victorian Infectious Diseases Reference Laboratory (Australia), or other in\u2010house or commercial assays already in use at their laboratories.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>An in silico analysis of isolates showed a good match to assay primer\/probes. The panel was distributed to 28 laboratories. Isolates were correctly identified in 98% of samples for detection and 99.6% for subtyping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The WHO RSV EQA 2019\u20132020 showed that laboratories performed at high standards. Updating the composition of RSV molecular EQAs with contemporary strains to ensure representation of circulating strains, and ensuring primer matching with EQA panel viruses, is advantageous in assessing diagnostic competencies of laboratories. Ongoing EQAs are recommended because of continued evolution of mismatches between current circulating strains and existing primer sets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.13073","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T21:29:12Z","timestamp":1674077352000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Results from the second WHO external quality assessment for the molecular detection of respiratory syncytial virus, 2019\u20132020"],"prefix":"10.1111","volume":"17","author":[{"given":"Thomas","family":"Williams","sequence":"first","affiliation":[{"name":"University of Edinburgh  Edinburgh UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1042-8882","authenticated-orcid":false,"given":"Sandra","family":"Jackson","sequence":"additional","affiliation":[{"name":"World Health Organization  Geneva 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Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Lindstrom","sequence":"additional","affiliation":[{"name":"Respiratory Virus Branch, Division of Viral Diseases Centers for Disease Control and Prevention  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Peret","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine University of Texas Medical Branch  Galveston Texas USA"},{"name":"Institute for Human Infections and Immunity University of Texas Medical Branch  Galveston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjiv","family":"Rughooputh","sequence":"additional","affiliation":[{"name":"United Kingdom Health Security Agency (UKHSA)  London UK"},{"name":"United Kingdom National External Quality Assessment Service (UK NEQAS) for Microbiology  London 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