{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:46:59Z","timestamp":1772160419176,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,14]],"date-time":"2022-12-14T00:00:00Z","timestamp":1670976000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["75N93021C00016"],"award-info":[{"award-number":["75N93021C00016"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001692","name":"Croucher Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001692","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Measures for mitigation of Coronavirus Disease 2019 (COVID\u201019) were set to reduce the spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS\u2010CoV\u20102). SARS\u2010CoV\u20102 and other respiratory viruses share similar transmission routes and some common clinical manifestations. Co\u2010circulation of SARS\u2010CoV\u20102 and other common respiratory viruses is imminent. Therefore, development of multiplex assays for detecting these respiratory viruses is essential for being prepared for future outbreaks of respiratory viruses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A panel of three reverse transcription droplet digital PCR (RT\u2010ddPCR) assays were developed to detect 15 different human respiratory viruses. Evaluations of its performance were demonstrated. A total of 100 local and 98 imported COVID\u201019 cases in Hong Kong were screened for co\u2010infection with other common respiratory viruses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All detected viral targets showed distinct signal clusters using the multiplex RT\u2010ddPCR assays. These assays have a broad range of linearity and good intra\u2010\/inter\u2010assay reproducibility for each target. The lower limits of quantification for all targets were \u226446 copies per reaction. Six imported cases of COVID\u201019 were found to be co\u2010infected with other respiratory viruses, whereas no local case of co\u2010infection was observed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The multiplex RT\u2010ddPCR assays were demonstrated to be useful for screening of respiratory virus co\u2010infections. The strict preventive measures applied in Hong Kong may be effective in limiting the circulation of other human respiratory viruses. The multiplex assays developed in this study can achieve a robust detection method for clinical and research purposes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.13084","type":"journal-article","created":{"date-parts":[[2022,12,15]],"date-time":"2022-12-15T00:41:57Z","timestamp":1671064917000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Development of multiplex RT\u2010ddPCR assays for detection of SARS\u2010CoV\u20102 and other common respiratory virus infections"],"prefix":"10.1111","volume":"17","author":[{"given":"Nathaniel K. C.","family":"Leong","sequence":"first","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haogao","family":"Gu","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisy Y. M.","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lydia D. J.","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavithra","family":"Krishnan","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel S. M.","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malik","family":"Peiris","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"},{"name":"Centre for Immunology and Infection Hong Kong Science and Technology Park  Hong Kong"},{"name":"HKU\u2010Pasteur Research Pole, School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9101-7953","authenticated-orcid":false,"given":"Leo L. M.","family":"Poon","sequence":"additional","affiliation":[{"name":"School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"},{"name":"Centre for Immunology and Infection Hong Kong Science and Technology Park  Hong Kong"},{"name":"HKU\u2010Pasteur Research Pole, School of Public Health, LKS Faculty of Medicine The University of Hong Kong  Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,12,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20102600(22)00142\u20104"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2022.06.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985\u2010021\u201001545\u20109"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00163\u201021"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985\u2010021\u201001594\u20100"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12769"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102672.2008.04119.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00310\u201020"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00563\u201008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.22186"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00848\u201007"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00993\u201009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.981\u2010986.2004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26749"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00108"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa029"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/2472630317705680"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201028420\u20107"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28066"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2021.2021806"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.03988\u20102020"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(22)00383\u2010X"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13084","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.13084","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,19]],"date-time":"2023-08-19T17:24:38Z","timestamp":1692465878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.13084"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,14]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/irv.13084"],"URL":"https:\/\/doi.org\/10.1111\/irv.13084","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/IRV.13084\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13084\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13084\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13084\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13084\/v2\/response1","asserted-by":"object"}]},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,14]]},"assertion":[{"value":"2022-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13084"}}