{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T22:18:54Z","timestamp":1775945934792,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T00:00:00Z","timestamp":1678320000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["19im0210112"],"award-info":[{"award-number":["19im0210112"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["22he2302005h0004"],"award-info":[{"award-number":["22he2302005h0004"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>There is a need for vaccines that can induce effective systemic, respiratory mucosal, and cellular immunity to control the COVID\u201019 pandemic. We reported previously that a synthetic mucosal adjuvant SF\u201010 derived from human pulmonary surfactant works as an efficient antigen delivery vehicle to antigen presenting cells in the respiratory and gastrointestinal tracts and promotes induction of influenza virus antigen\u2010specific serum IgG, mucosal IgA, and cellular immunity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The aim of the present study was to determine the effectiveness of a new administration route of trans\u2010airway (TA) vaccine comprising recombinant SARS\u2010CoV\u20102 spike protein 1 (S1) combined with SF\u201010 (S1\u2010SF\u201010 vaccine) on systemic, local, and cellular immunity in mice, compared with intramuscular injection (IM) of S1 with a potent adjuvant AddaS03\u2122 (S1\u2010AddaS03\u2122 vaccine).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>S1\u2010SF\u201010\u2010TA vaccine induced S1\u2010specific IgG and IgA in serum and lung mucosae. These IgG and IgA induced by S1\u2010SF\u201010\u2010TA showed significant protective immunity in a receptor binding inhibition test of S1 and angiotensin converting enzyme 2, a receptor of SARS\u2010CoV\u20102, which were more potent and faster achievement than S1\u2010AddaS03\u2122\u2010IM. Enzyme\u2010linked immunospot assay showed high numbers of S1\u2010specific IgA and IgG secreting cells (ASCs) and S1\u2010responsive IFN\u2010\u03b3, IL\u20104, IL\u201017A cytokine secreting cells (CSCs) in the spleen and lungs. S1\u2010AddaS03\u2122\u2010IM induced IgG ASCs and IL\u20104 CSCs in spleen higher than S1\u2010SF\u201010\u2010TA, but the numbers of ASCs and CSCs in lungs were low and hardly detected.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Based on the need for effective systemic, respiratory, and cellular immunity, the S1\u2010SF\u201010\u2010TA vaccine seems promising mucosal vaccine against respiratory infection of SARS\u2010CoV\u20102.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.13119","type":"journal-article","created":{"date-parts":[[2023,3,9]],"date-time":"2023-03-09T04:31:16Z","timestamp":1678336276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Induction of systemic, mucosal, and cellular immunity against SARS\u2010CoV\u20102 in mice vaccinated by trans\u2010airway with a S1 protein combined with a pulmonary surfactant\u2010derived adjuvant SF\u201010"],"prefix":"10.1111","volume":"17","author":[{"given":"Takashi","family":"Kimoto","sequence":"first","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoko","family":"Sakai","sequence":"additional","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"},{"name":"Faculty of Pharmaceutical Sciences Tokushima University  Tokushima Japan"},{"name":"Institute for Genome Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Kameda","sequence":"additional","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Morita","sequence":"additional","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Etsuhisa","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuo","family":"Shinohara","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Tokushima University  Tokushima Japan"},{"name":"Institute for Genome Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4320-7285","authenticated-orcid":false,"given":"Hiroshi","family":"Kido","sequence":"additional","affiliation":[{"name":"Division of Enzyme Chemistry, Institute for Enzyme Research Tokushima University  Tokushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,9]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"World Health Organization.Coronavirus disease (COVID\u201019) dashboard. Accessed February 13 2023.https:\/\/covid19.who.int"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2034577"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2035389"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(20)32661\u20101"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2115481"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1213"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1101\/2021.03.11.434841"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.744887"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2021.103788"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0169\u2010409x(01)00162\u20104"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15217"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13526"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr7005a1"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.11.030"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.2.1122"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.024"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.05.090"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12124"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.12.002"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.02.061"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191133"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003530\u20102"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473\u20103099(21)00147\u2010X"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc2241"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abd2223"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108728"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001002\u20101"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2552"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200955"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201017292\u20104"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10061550"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1539"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13119","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.13119","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13119","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T12:42:28Z","timestamp":1692621748000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.13119"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":32,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/irv.13119"],"URL":"https:\/\/doi.org\/10.1111\/irv.13119","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/IRV.13119\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13119\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13119\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13119\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13119\/v1\/review1","asserted-by":"object"}]},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3]]},"assertion":[{"value":"2022-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13119"}}