{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:47:03Z","timestamp":1772160423254,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,11]],"date-time":"2024-07-11T00:00:00Z","timestamp":1720656000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Amid coronavirus disease 2019 (COVID\u201019) pandemic, accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) is critical for diagnosis management and breaking down transmission chains. We designed a national external quality assessment panel (EQAP) for SARS\u2010CoV\u20102 molecular detection comprising working laboratories nationwide.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A molecular diagnostic EQA panel that consists of five samples for SARS CoV\u20102 testing was distributed to 141 public and private sector laboratories across country. These samples contain different concentrations of SARS\u2010CoV\u20102 to evaluate the sensitivity of commercial kits available.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Sensitivity among public and private sector laboratories was variable, particularly lower SARS\u2010CoV\u20102 concentrations significantly increased the risk of false\u2010negative tests, whereas Ct values of accurately tested SARS\u2010CoV\u20102 specimens increased as concentration decreased. These findings highlighted that performance of used commercial kits was not significantly correlated to various extraction or PCR methods.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study highlights the need for a national external quality assessment panel (EQAP) in the country to improve the quality of the healthcare system while ensuring the accuracy and reliability of results. Furthermore, EQAPs can help laboratories meet accreditation and regulatory requirements. However, continued participation in EQAP is recommended for quality enhancement of laboratories.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.13316","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T01:39:01Z","timestamp":1720748341000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["External Quality Assessment Program for SARS\u2010COV\u20102 Molecular Detection in Pakistan"],"prefix":"10.1111","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2329-7877","authenticated-orcid":false,"given":"Nazish","family":"Badar","sequence":"first","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aamer","family":"Ikram","sequence":"additional","affiliation":[{"name":"Executive Director Office National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Salman","sequence":"additional","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sidra","family":"Saeed","sequence":"additional","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamza\u00a0Ahmed","family":"Mirza","sequence":"additional","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7953-0458","authenticated-orcid":false,"given":"Abdul","family":"Ahad","sequence":"additional","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asiya","family":"Ashraf","sequence":"additional","affiliation":[{"name":"Public Health Laboratories Division National Institute of Health  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umer","family":"Farooq","sequence":"additional","affiliation":[{"name":"Animal Sciences Institute National Agricultural Research Center  Islamabad Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,7,11]]},"reference":[{"key":"e_1_2_10_2_1","unstructured":"WHO Coronavirus (COVID\u201019) Dashboard April 28 2023 https:\/\/covid19.who.int."},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa760"},{"key":"e_1_2_10_4_1","unstructured":"World Health Organization (WHO) \u201cCOVID\u201019 Target Product Profiles for Priority Diagnostics to Support Response to the COVID\u201019 Pandemic v.1.0 \u201d no.September (2020) 1\u201338."},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa099"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2610.202551"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-020-0754-0"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2608.201843"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-0569"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096\u2010020\u201003913\u20109"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.04.083"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","unstructured":"B.Bergmans C. B.Reusken A. J.vanOudheusden et\u00a0al. \u201cDeclining SARS\u2010CoV\u20102 PCR Sensitivity With Time and Dependence on Clinical Features: Consequences for Control \u201d (2020) medRxiv.","DOI":"10.1101\/2020.08.23.20179408"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560\u20107917.ES.2020.25.27.2001223"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02676\u201020"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1214\/aoms\/1177731363"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics12061483"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102196\u2010x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.12.094"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00683-21"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104537"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T10:11:37Z","timestamp":1721988697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.13316"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/irv.13316"],"URL":"https:\/\/doi.org\/10.1111\/irv.13316","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/IRV.13316\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13316\/v2\/decision1","asserted-by":"object"}]},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2023-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13316"}}