{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T22:56:40Z","timestamp":1784761000725,"version":"3.55.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T00:00:00Z","timestamp":1719187200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","award":["21HA2003"],"award-info":[{"award-number":["21HA2003"]}],"id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP18K10036"],"award-info":[{"award-number":["JP18K10036"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP233fa627001"],"award-info":[{"award-number":["JP233fa627001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Influenza viruses can cause zoonotic infections that pose public health risks. Surveillance of influenza A and B viruses is conducted globally; however, information on influenza C and D viruses is limited. Longitudinal monitoring of influenza C virus in humans has been conducted in several countries, but there has been no long\u2010term monitoring of influenza D virus in humans. The public health risks associated with the influenza D virus therefore remain unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established a duplex real\u2010time RT\u2010PCR to detect influenza C and D viruses and analyzed respiratory specimens collected from 2144 patients in Japan with respiratory diseases between January 2018 and March 2023. We isolated viruses and conducted hemagglutination inhibition tests to examine antigenicity and focus reduction assays to determine susceptibility to the cap\u2010dependent endonuclease inhibitor baloxavir marboxil.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We detected three influenza C viruses belonging to the C\/Kanagawa\u2010 or C\/Sao Paulo\u2010lineages, which recently circulated globally. None of the specimens was positive for the influenza D virus. The C\/Yokohama\/1\/2022 strain, isolated from the specimen with the highest viral RNA load and belonging to the C\/Kanagawa\u2010lineage, showed similar antigenicity to the reference C\/Kanagawa\u2010lineage strain and was susceptible to baloxavir.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our duplex real\u2010time RT\u2010PCR is useful for the simultaneous detection of influenza C and D viruses from the same specimen. Adding the influenza D virus to the monitoring of the influenza C virus would help in assessing the public health risks posed by this virus.<\/jats:p><\/jats:sec>","DOI":"10.1111\/irv.13345","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T05:15:37Z","timestamp":1719292537000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Monitoring Influenza C and D Viruses in Patients With Respiratory Diseases in Japan, January 2018 to March 2023"],"prefix":"10.1111","volume":"18","author":[{"given":"Kohei","family":"Shimizu","sequence":"first","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiharu","family":"Kawakami","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"},{"name":"Pandemic Preparedness, Infection, and Advanced Research Center The University of Tokyo  Tokyo Japan"},{"name":"Research Center for Global Viral Diseases National Center for Global Health and Medicine Research Institute  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Yamagata University Faculty of Medicine  Yamagata Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiichiro","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiho","family":"Nagata","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Morita","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayo","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideka","family":"Miura","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Momoki","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miwako","family":"Saikusa","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Ozawa","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Kumazaki","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuzo","family":"Usuku","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuko","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryuichi","family":"Senda","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Okubo","sequence":"additional","affiliation":[{"name":"Yokohama City Institute of Public Health  Yokohama Kanagawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Kawaoka","sequence":"additional","affiliation":[{"name":"Pandemic Preparedness, Infection, and Advanced Research Center The University of Tokyo  Tokyo Japan"},{"name":"Research Center for Global Viral Diseases National Center for Global Health and Medicine Research Institute  Tokyo Japan"},{"name":"Influenza Research Institute, Department of Pathobiological Sciences, School of Veterinary Medicine University of Wisconsin\u2010Madison  Madison Wisconsin USA"},{"name":"Division of Virology, Institute of Medical Science The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9064-4699","authenticated-orcid":false,"given":"Emi","family":"Takashita","sequence":"additional","affiliation":[{"name":"Research Center for Influenza and Respiratory Viruses National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.2.422-429.2002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/502973"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2023.105429"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003176"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10121583"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12402"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28201"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01051-20"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01928-21"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2017.08.005"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2014.06.017"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MRA.00900-21"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31828fca10"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix471"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2016.05.017"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12010030"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2018.00174"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-018-0071-7"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-020-0105-9"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/zph.13008"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0201295"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2015.11.036"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2012.02.012"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14071422"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10080417"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15010244"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2510.190607"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/irv.12854"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13345","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T18:53:33Z","timestamp":1720551213000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.13345"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":28,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/irv.13345"],"URL":"https:\/\/doi.org\/10.1111\/irv.13345","archive":["Portico"],"relation":{},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"2024-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13345"}}